Quantibody® Human Cytokine Array 1000 Kit. A combination of Human Inflammation Array Q3 and Human Growth Factor Array Q1. Detects 80 Human Inflammatory and Growth Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 80 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application
|
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Amphiregulin
| BDNF
| beta-NGF
| bFGF
| BLC (CXCL13)
|
BMP-4
| BMP-5
| BMP-7
| EGF
| EGFR
|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| FGF-4
| FGF-7 (KGF)
| GCSF
|
GDF-15
| GDNF
| GM-CSF
| Growth Hormone
| EG-VEGF (PK1)
|
HB-EGF
| HGF
| I-309 (TCA-3/CCL1)
| ICAM-1 (CD54)
| IFN-gamma
|
IGF-1
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
IGFBP-6
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
| IL-10
|
IL-11
| IL-12 p40
| IL-12 p70
| IL-13
| IL-15
|
IL-16
| IL-17A
| IL-2
| IL-4
| IL-5
|
IL-6
| IL-6 R
| IL-7
| IL-8 (CXCL8)
| Insulin
|
MCP-1 (CCL2)
| M-CSF
| M-CSF R
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| NGFR (TNFRSF16)
| NT-3
| NT-4
|
Osteoprotegerin (TNFRSF11B)
| PDGF-AA
| PDGF-BB
| PLGF
| RANTES (CCL5)
|
SCF
| SCF R (CD117/c-kit)
| TGF alpha
| TGF beta 1
| TGF beta 3
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
|
TNF RII (TNFRSF1B)
| VEGF-A
| VEGF-D
| VEGFR2
| VEGFR3
|
Species Detected
Human
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Human Cytokine Array Q1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Ciavarella S., Caselli A., Tamma A., et al. A peculiar molecular profile of umbilical cord-mesenchymal stromal cells drives their inhibitory effects on multiple myeloma cell growth and tumor progression. Stem Cells Dev. 2015 Jun 15;24(12):1457-70. doi: 10.1089/scd.2014.0254.
Species:
Human
Sample Type:
Conditioned Media (MSC, umbilical and bone marrow stem cell cultures)
Massee M., Chinn K., Lei J., et al. Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro. J Biomed Mater Res B Appl Biomater. 2015 Jul 14. doi: 10.1002/jbm.b.33478
Species:
Human
Sample Type:
Conditioned Media (ADCS, BM-MSCs and HSCs stem cell lines)
Massee M., Chinn K., Lim J., et al. Type I and II Diabetic Adipose-Derived Stem Cells Respond In Vitro to Dehydrated Human Amnion/Chorion Membrane Allograft Treatment by Increasing Proliferation, Migration, and Altering Cytokine Secretion. Adv Wound Care (New Rochelle). 2016 Feb 1;5(2):43-54
Species:
Human
Sample Type:
Conditioned Media (Adipose derived stem cells with or without dHCAM)
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