RayBio® Human ICAM-1 (CD54) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

As low as $420.00
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Catalog #:
ELH-ICAM1
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Human
Accession Number P05362
Gene Id 3383
Gene Symbols ICAM1
Protein Name / Synonyms Intercellular adhesion molecule 1 (ICAM-1) (Major group rhinovirus receptor) (CD antigen CD54)
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Research Area Antibody Drug Development, Protein Therapeutics, Biomarker Detection
Sensitivity 150 pg/ml

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Detection Range 150 pg/ml - 20 ng/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Li, Y., Sun, B., Shi, Y., Jiang, J., Du, Z., Chen, R., Sun, Z. (2020). Subacute exposure of PM2.5 induces airway inflammation through inflammatory cell infiltration and cytokine expression in rats. Chemosphere, 251, 126423. doi:10.1016/j.chemosphere.2020.126423 
Species:
Human
Sample Type:
Conditioned media (cell culture media, BEAS-2B )
Chen, Xia, et al. "Lanthanum chloride inhibits LPS mediated expressions of pro-inflammatory cytokines and adhesion molecules in HUVECs: involvement of NF-?B-Jmjd3 signaling." Cellular Physiology and Biochemistry 42.5 (2017): 1713-1724.
Species:
Human
Sample Type:
Conditioned Media
Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 - 1883
Species:
Human
Sample Type:
Serum
Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
Species:
Human
Sample Type:
Serum (Lupus Nephritis patients)
Tammali, Ravinder, et al. "Inhibition of aldose reductase prevents angiogenesis in vitro and in vivo." Angiogenesis 14.2 (2011): 209-221.
Species:
Human
Sample Type:
Conditioned Media
Nagaraja V, Spino C, Bush E, Tsou PS, Domsic RT, Lafyatis R, Frech T, Gordon JK, Steen VD, Khanna D. A multicenter randomized, double-blind, placebo-controlled pilot study to assess the efficacy and safety of riociguat in systemic sclerosis-associated digital ulcers. Arthritis Res Ther. 2019 Sep 3;21(1):202. doi: 10.1186/s13075-019-1979-7. PMID: 31481106; PMCID: PMC6724329.
Species:
Human
Sample Type:
Plasma (patients)
Liu, Zhao-Min, et al. "Effect of soy protein and isoflavones on blood pressure and endothelial cytokines: a 6-month randomized controlled trial among postmenopausal women." Journal of hypertension 31.2 (2013): 384-392.
Species:
Human
Sample Type:
Serum (postmenopausal women with pre or early diabetes w)
Bala, Kanchan, Kiran Ambwani, and Nivedita Karmakar Gohil. "Effect of different mitogens and serum concentration on HUVEC morphology and characteristics: Implication on use of higher passage cells." Tissue and Cell 43.4 (2011): 216-222.
Species:
Human
Sample Type:
Cell Lysate (HUVEC)
Chao, Pi-Yu, Ya-Ping Huang, and Wen-Bin Hsieh. "Inhibitive effect of purple sweet potato leaf extract and its components on cell adhesion and inflammatory response in human aortic endothelial cells." Cell adhesion & migration 7.2 (2013): 237-245.
Species:
Human
Sample Type:
Conditioned Media (effects of pytochemicals and aspirin on the HAECs surface expression)
Ro, Han, et al. "Roles of islet Toll-like receptors in pig to mouse islet xenotransplantation." Cell transplantation 22.9 (2013): 1709-1722.
Species:
Porcine
Sample Type:
Conditioned Media (pig islet cells)
  1. Great Product
    Rating
    100%
    This kit worked very well for my cell culture supernatant samples. The protocol was detailed and clear to follow. Would definitely recommend.

    Review by

    from University of Georgia,

    on

  2. Great product
    Rating
    100%
    I recommend the ELISA kits from RayBiotech. Easy to follow with cell culture supernatant.

    Review by

    from Florida A&M University,

    on

  3. Great product
    Rating
    100%
    Very sensitive assay system at great price. Highly recommended.

    Review by

    from University of South Alabama,

    on

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