Phosphorylation Assays
Phosphorylation, a fundamental process in cellular signaling, involves the addition of phosphate groups to proteins, regulating their activity, localization, and interactions within the cell. Whether you're aiming for precise quantitative data or qualitative insights into phosphorylation states, our collection offers sensitive and user-friendly cell-based or in vitro assays to suit your needs. Opt for our ELISA format for high-throughput quantitative analyses on individual targets or explore our array format for simultaneous assessment of multiple targets.

Navigate Cell Signaling Pathways using Phosphorylation Assays
Explore RayBiotech's expansive range of phosphorylation assays, including ELISAs, arrays, and functional assay kits tailored for human, mouse, and rat species. Our phosphorylation assays cover a diverse array of cell signaling pathways, targeting key pathways such as, MAPK, PI3-AKT, HER/ErbB, JAK/STAT, NF-kB. Our assay kits offer versatility and compatibility with various sample types including cell lysates, tissue lysates, adherent cell culture, and more.
Additionally, our phosphorylation arrays provide rapid, sensitive assays for simultaneous detection of multiple protein phosphorylation, enabling efficient pathway activation verification without the need for time-consuming methods like immunoprecipitation or Western Blot analysis. Trust RayBiotech for precise solutions to navigate complex cellular signaling pathways and accelerate your research endeavors.
Popular phosphorylation assays
- Phospho-Tau (T181) Quantitative ELISA Kit
Phospho-Tau (Thr181) ELISA Kit for the quantitative measurement of phosphorylated Tau at Threonine-181 in human, mouse, or rat lysate samples.
- Phospho-Tau (Ser202/Thr205) Quantitative ELISA Kit The RayBiotech Phospho-Tau (Ser202/Thr205) ELISA is a phosphorylation assay kit for the quantitative measurement of phosphorylated Tau at Ser202/Thr205 in Human, Mouse and Rat serum, plasma and CSF.
- Phospho-AKT (S473) ELISA Kit
RayBio® Human/Mouse/Rat Phospho-Akt (S473) ELISA Kit. This assay semi-quantitatively measures phosphorylated Akt (Ser473) in lysate samples.
- Human Phospho-H2AX (S139) ELISA Kit
RayBio® Human Phospho-H2AX (S139) ELISA Kit. This assay semi-quantitatively measures phosphorylated H2AX (Ser139) in lysate samples.
- Human Phospho-eNOS (S1177) ELISA Kit
RayBio® Human Phospho-eNOS (S1177) ELISA Kit. This assay semi-quantitatively measures phosphorylated eNOS (Ser1177) in lysate samples.
- Phospho-Stat 3 (Y705) ELISA Kit
RayBio® Human/Mouse/Rat Phospho-Stat 3 (Y705) ELISA Kit. This assay semi-quantitatively measures phosphorylated Stat 3 (Tyr705) in lysate samples.
- Phospho-Stat 1 (Y701) and Total Stat 1 ELISA Kit
RayBio® Human/Mouse Phospho-Stat 1 (Y701) and Total Stat 1 ELISA Kit. This assay semi-quantitatively measures phosphorylated Stat 1 (Tyr701) and Total Stat 1 in lysate samples.
- Phospho-mTOR (S2448) ELISA Kit
RayBio® Human and Mouse Phospho-mTOR (Ser2448) ELISA Kit. This assay semi-quantitatively measures mTOR phosphorylated at Serine-2448 in cell lysate samples.
- Phospho-NF-KB p65 (S536) ELISA Kit
RayBio® Human/Mouse/Rat Phospho-NF-KB p65 (S536) ELISA Kit. This assay semi-quantitatively measures phosphorylated NF-KB p65 (Ser536) in lysate samples.
- Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit
RayBio® Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (T185/Y187) ELISA Kit. This assay semi-quantitatively measures phosphorylated Erk1 (Thr202/Tyr204) + in lysate samples.
New Products
- Phospho-TNK1 (Tyr277) ELISA Kit The RayBiotech Phospho-TNK1 (Tyr277) ELISA Kit is a convenient and sensitive assay to detect phosphorylated TNK1 at Tyrosine-277 in human cell lysate samples.
- Phospho-TNK1 (Tyr277) and Total TNK1 ELISA Kit The RayBiotech Phospho-TNK1 (Tyr277) and Total TNK1 ELISA Kit is a convenient and sensitive assay to detect both phosphorylated TNK1 at Tyrosine-277 and total (pan) TNK1 in human cell lysate samples.
- Phospho-IRS1 (Ser318) and Total IRS1 ELISA Kit The RayBio® Phospho-IRS1 (Ser318) and Total IRS1 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates.
- Phospho-IRS1 (Ser318) ELISA Kit The RayBio® Phospho-IRS1 (Ser318) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates.
- Human Phosphotyrosine JAK3 ELISA Kit
RayBio® Human Phosphotyrosine JAK3 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine JAK3 in lysate samples. 1 - 2 business days lead time.
- Phospho-CDC25A (S293) and Total CDC25A ELISA Kit The RayBio® Human and Mouse Phospho-CDC25A (S293) and Total CDC25A ELISA Kit semi-quantitatively measures phosphorylated CDC25A (Ser293) and total CDC25A in lysate samples.
- Phospho-CDC25A (S293) ELISA Kit The RayBio® Human and Mouse Phospho-CDC25A (S293) ELISA Kit semi-quantitatively measures phosphorylated CDC25A (Ser293) in lysate samples.
- Human Phospho-CHK1 (S296) and Total CHK1 ELISA Kit The RayBio® Human Phospho-CHK1 (S296) and Total CHK1 ELISA Kit semi-quantitatively measures phosphorylated CHK1 (Ser296) and Total CHK1 in lysate samples.
- Human Phospho-CHK1 (S296) ELISA Kit The RayBio® Human Phospho-CHK1 (S296) ELISA Kit semi-quantitatively measures phosphorylated CHK1 (Ser296) in lysate samples.
What customers are saying
“The Phospho-PTEN (S380)/ total PTEN ELISA [CAT# PEL-PTEN-S380-T] works very well and gives repetitive and accurate results with lysates from cancer cell lines.”
Marina
Australis Pharmaceuticals

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