RayBio® Human HB-EGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

As low as $600.00
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Catalog #:
ELH-HBEGF
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Human
Accession Number Q99075
Gene Id 1839
Gene Symbols HBEGF|DTR|DTS|HEGFL
Protein Name / Synonyms Proheparin-binding EGF-like growth factor [Cleaved into: Heparin-binding EGF-like growth factor (HB-EGF) (HBEGF) (Diphtheria toxin receptor) (DT-R)]
Quantitative/Semi-Quantitative Quantitative
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Sensitivity 20 pg/ml

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Detection Range 20 pg/ml - 4000 pg/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Lim Y., Yoo J., Kim M., et al. GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands. Mol Cancer Ther. 2016 Feb;15(2):251-63. doi: 10.1158/1535-7163.MCT-15-0679
Species:
Human
Sample Type:
Conditioned Media (Tumor xenografts)
David M., Sahay D., Mege F., Descotes F., Leblanc R., Ribeiro J., et al. Identification of Heparin-Binding EGF-Like Growth Factor (HB-EGF) as a Biomarker for Lysophosphatidic Acid Receptor Type 1 (LPA1) Activation in Human Breast and Prostate Cancers. PLOS One 9(5): e97771.
Species:
Mouse
Sample Type:
Serum
Xu X, Gurski LA, Zhang C, Harrington DA, Farach-Carson MC, Jia X. Recreating the Tumor Microenvironment in a Bilayer, Hyaluronic Acid Hydrogel Construct for the Growth of Prostate Cancer Spheroids. Biomaterials 2012;33(35):9049-9060. doi:10.1016/j.biomaterials.2012.08.061.
Species:
Human
Sample Type:
Conditioned Media (HBEGF releasing system, hydrogel particles)
Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
Species:
Human
Sample Type:
Tissue Lysate (Dehydrated Human Amnion/Chorion Membrane Allografts)
Rayego?Mateos, Sandra, et al. "TWEAK transactivation of the epidermal growth factor receptor mediates renal inflammation." The Journal of pathology 231.4 (2013): 480-494.
Species:
Human
Sample Type:
Tissue Lysate (kidney )
  1. human HBEGF in cell culture supernatants
    Rating
    100%
    The kit was easy to use and the instructions were straightforward.
    We used this on human endothelial cell culture supernatants and were able to detect low levels in our undiluted samples (~15-60pg/mL). The standard curve looked great, but in the future we may concentrate our samples to get in the middle of the curve.

    Review by

    from Massachusetts general hospital,

    on

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