Human IGFBP-1 ELISA Kit

Name: Human IGFBP-1 ELISA Kit
Brand: ELH-IGFBP1
SKU: ELH-IGFBP1
Price: 420 USD
Availability: InStock
Rating: 100 (3 reviews)

Rating:

100 % of 100

3 Reviews

11 Citations

RayBio® Human IGFBP-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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As low as $420.00

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Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human
Accession Number	P08833
Gene Id	3484
Gene Symbols	IGFBP1\|IBP1
Protein Name / Synonyms	Insulin-like growth factor-binding protein 1 (IBP-1) (IGF-binding protein 1) (IGFBP-1) (Placental protein 12) (PP12)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	5 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	5 pg/ml - 2000 pg/ml
Recommended Dilution (Serum/Plasma)	5 - 500 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELH-IGFBP1.pdf)

SDS (ELH-IGFBP1.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Farschtschi, S., Kluwe, L., Park, SJ. et al. Upregulated immuno-modulator PD-L1 in malignant peripheral nerve sheath tumors provides a potential biomarker and a therapeutic target. Cancer Immunol Immunother 69, 1307-1313 (2020). https://doi.org/10.1007/s00262-020-02548-4

Species:

Human

Sample Type:

Serum

View Publication

Wright, Jonathan L., et al. "A study of caloric restriction versus standard diet in overweight men with newly diagnosed prostate cancer: a randomized controlled trial." The Prostate73.12 (2013): 1345-1351.

Species:

Human

Sample Type:

Serum (caloric restriction versus standard diet in overweight men with newly diagnosed prostate cancer:)

View Publication

Pflum ZE, Palumbo SL, Li W-J. Adverse effect of demineralized bone powder on osteogenesis of human mesenchymal stem cells. Exp Cell Res. 2013 Aug 1;319(13):1942-1955.

Species:

Human

Sample Type:

Conditioned Media (mesenchymal stem cells)

View Publication

Colostrum Supplementation Restores Insulin-like Growth Factor -1 Levels and Alters Muscle Morphology Following Massive Small Bowel Resection

Species:

Human

View Publication

Estella C, Herrer I, Moreno-Moya JM, et al. miRNA Signature and Dicer Requirement during Human Endometrial Stromal Decidualization In Vitro. Knott JG, ed. PLoS ONE. 2012;7(7):e41080. doi:10.1371/journal.pone.0041080.

Species:

Human

Sample Type:

Conditioned Media (Human endometrial Stromal Cells)

View Publication

Ho, Huiting, et al. "Small molecule proprotein convertase inhibitors for inhibition of embryo implantation." PloS one 8.12 (2013): e81380.

Species:

Human

Sample Type:

Conditioned Media (Human endometrial stromal cells (HESCs) )

View Publication

Founds S., Zeng X., Lykins D., and Robers J.

Species:

Human

Sample Type:

Serum (Preeclampsia biomarker patients)

View Publication

Pineda M., Lu Z., Cao D., Kim J. Influence of Cancer-Associated Endometrial Stromal Cells on Hormone-Driven Endometrial Tumor Growth. Horm Cancer. 2015 Aug;6(4):131-41. doi: 10.1007/s12672-015-0223-4

Species:

Human

Sample Type:

Conditioned Media (Endometrial stromal cells secretions)

View Publication

Wu X, Zheng W, Jin P, Hu J, Zhou Q. Role of IGFBP1 in the senescence of vascular endothelial cells and severity of aging?related coronary atherosclerosis. Int J Mol Med. 2019 Nov;44(5):1921-1931. doi: 10.3892/ijmm.2019.4338. Epub 2019 Sep 12. PMID: 31545483; PMCID: PMC6777673.

Species:

Human

Sample Type:

Conditioned Media

View Publication

Aucouturier J, Thivel D, Isacco L, et al. Combined food intake and exercise unmask different hormonal responses in lean and obese children. Appl Physiol Nutr Metab. 2013;38(6): 638-643

Species:

Human

Sample Type:

Plasma (purchased from Clinisciences (Montrouge, France))

View Publication

View All Publications For This Product

Reviews

Write a customer review

Great product

Rating
100%

Simple and Fast

Review by K,

from NIH,

on Jul 13, 2020
Great product

Rating
100%

SerumVery good result

Review by Kim Han,

from NIH,

on Jul 2, 2019
Great product

Rating
100%

SerumVery good result

Review by Kim Han,

from NIH,

on Jan 2, 2019

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Human IGFBP-1 ELISA Kit