The RayBiotech BDNF ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, and cell culture supernatant samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Human |
| Accession Number | P23560 |
| Gene Id | 627 |
| Gene Symbols | BDNF |
| Protein Name / Synonyms | BDNF |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN- gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF- beta, TIMP-1, TIMP-2, TNF- alpha, TNF- beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 80 pg/ml Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 80 pg/ml - 16 ng/ml |
| Recommended Dilution (Serum/Plasma) | 5 - 500 fold |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Related Products
| Sample Type | Average % Recovery | Range (%) |
|---|---|---|
| Serum | 94.57 | 83-103 |
| Plasma | 92.48 | 84-102 |
| Cell culture media | 93.35 | 83-102 |
Linearity Results
| Â | Â | Serum | Plasma | Cell Culture Media |
|---|---|---|---|---|
| 1:2 | Average % of expected Range (%) |
93 83-103 |
92 84-103 |
94 83-104 |
| 1:4 | Average % of expected Range (%) |
94 84-104 |
93 83-102 |
95 85-103 |
Application Notes
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
Storage/Stability
Wang X., Prager B., Wu Q., et al. Reciprocal Signaling between Glioblastoma Stem Cells and Differentiated Tumor Cells Promotes Malignant Progression. Cell Stem Cell . 2018 Apr 5;22(4):514-528.e5. doi: 10.1016/j.stem.2018.03.011.
prep tips we pri
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Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 - 1883
Liu Y., et al. Associations Between ApoE?4 Carrier Status and Serum BDNFLevels--New Insights into the Molecular Mechanism of ApoE?4. Mol Neurobio. Online First June 2014. DOI 10.1007/s12035-014-8804-8Actions in Alzheimer's Disease
Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
Failla M., Conley Y., Wagner A. Brain-Derived Neurotrophic Factor (BDNF) in Traumatic Brain Injury-Related Mortality: Interrelationships Between Genetics and Acute Systemic and Central Nervous System BDNF Profiles. Neurorehabil Neural Repair. 2016 Jan;30(1):83-93. doi: 10.1177/1545968315586465.
Failla M., Conley Y., Wagner A. Brain-Derived Neurotrophic Factor (BDNF) in Traumatic Brain Injury-Related Mortality: Interrelationships Between Genetics and Acute Systemic and Central Nervous System BDNF Profiles. Neurorehabil Neural Repair. 2016 Jan;30(1):83-93. doi: 10.1177/1545968315586465.
Failla M., Juengst S., Arenth P., Wagner A. Preliminary Associations Between Brain-Derived Neurotrophic Factor, Memory Impairment, Functional Cognition, and Depressive Symptoms Following Severe TBI. Neurorehabil Neural Repair. 2016 Jun;30(5):419-30. doi: 10.1177/1545968315600525.
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Reliable AssayWe used serum and ran a dilution test using two samples diluted at 25X, 50X, 100X, and 200X. We selected 200X to use for our samples and have found no issues whatsoever. We've currently run this on 40 samples and expect to run 80 more in the coming weeks.
from University of Arizona,
on
Great product and performanceWe use serum samples diluted at 200X for this assay. Easy to use, standards are reliable, and seems reproducible.from University of Arizona,
on
Great productEasy to use with clear instructions! Especially for my first time using a comparable kit.from University of California,
on
Great productAmazing kit! Easy to use, reliable, convenient. Totally recommended.from NIA/NIH,
on
Great productTwo kits were purchased during a promotional sale, and were just used this week. The standard curve was perfect as compared to QC data. Although the samples were saturated (my fault with dilution), the kit worked properly! Looking forward to ordering again for our sample databases.from University of Northern Colorado,
on
Great productGreat directions and easy to use.from UNC,
on
Great productThe BDNF ELISA kit was easy to use and produced accurate results. The instructions were clear and the protocol was straight forward. I ran a series of 4 plates and did not have any issues.from University of Arkansas,
on
Great productHelped teach the lab assistants how to use it, worked out well. User-friendly and accurate.on
Great productThe BDNF ELISA kit was very easy to work with. I had no problems understanding the directions and following them. I used the kit once it arrived and received great results.on
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