Flow Cytometry Reagents
Gain quantitative single cell insights with flow cytometry and FACS reagents that come with the dependability and quality scientists expect from RayBiotech.
Manufactured in our US-based facility for supply chain reliability and backed by our expert technical support scientists, you can confidently advance a wide range of flow cytometry and FACS projects with RayBiotech.
Bright dyes, quality antibodies, reliable kits
Whether you’re looking for a tagged secondary antibody that can deliver low background binding and high signal-to-noise ratios, an unlabeled primary antibody, a cell viability marker that won’t overlap with your existing panel, or a convenient kit that includes a complete set of reagents, you can find it at RayBiotech.
A focus on quality
At RayBiotech, we understand how variable biology can be and strive for consistency in every product we manufacture. With our flow cytometry reagents, this is reflected in our quality control studies which include using flow cytometry to test every batch instead of faster, simpler western blots. This way we can verify performance in your intended application.
Flow Cytometry Reagent Categories
Popular Flow Cytometry Reagents
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Flow Cytometry Compensation BeadsThe RayBright® universal flow cytometry compensation beads capture all rat, mouse, Syrian hamster, Armenian hamster, and rabbit antibodies that are labeled with all current fluorophores.
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Anti-Human CD20 Antibody, Biotinylated Biotinylated Human CD20 Antibody, monoclonal Mouse IgG2a. Validated for flow cytometry. -
Anti-Mouse MHC class I Antibody, RayBright® Red 780 RayBright® Red 780 Mouse MHC class I Antibody [clone: M1/42], monoclonal Rat IgG2a kappa. Validated for flow cytometry. -
Anti-Human CD56 Antibody, Biotinylated Biotinylated Human CD56 Antibody [clone: 5.1H], monoclonal Mouse IgG1 kappa. Validated for flow cytometry. -
Anti-Mouse CD11c Antibody, Purified Mouse CD11c Antibody [clone: N418], monoclonal Hamster IgG kappa. Validated for flow cytometry. -
Anti-Human CD34 Antibody, Biotinylated Biotinylated Human CD34 Antibody, monoclonal Mouse . Validated for flow cytometry. -
Anti-Human CD25 Antibody, Biotinylated Biotinylated Human CD25 Antibody [clone: 7G7B6], monoclonal Mouse IgG2a kappa. Validated for flow cytometry. -
Anti-Human CD14 Antibody, Purified Human CD14 Antibody [clone: 26ic], monoclonal Mouse IgG2b kappa. Validated for flow cytometry. -
Anti-Human MHC class I Antibody, RayBright® Red 780 RayBright® Red 780 Human MHC class I Antibody [clone: W6/32], monoclonal Mouse IgG2a kappa. Validated for flow cytometry. -
Anti-Mouse CD205 Antibody, Purified Mouse CD205 Antibody [clone: DEC-205 [NLDC-145]], monoclonal Rat IgG2a. Validated for flow cytometry.
New Products
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TBMNK Flow Cytometry Assay KitThe RayBio® Human TBMNK Flow Cytometry Kit is designed to determine the percentages of mature human lymphocyte and monocyte subsets in peripheral whole blood (WB).
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Anti-Human CD45RO Antibody, RayBright® Red 780 Human CD45RO Antibody [clone: UCHL-1], monoclonal mouse IgG2a. Validated for flow cytometry. -
Anti-Human CD7 Antibody, RayBright® Red 780 Human CD7 Antibody [clone: T3-3A1], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
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Anti-Human CD11 C Antibody, RayBright® Red 780 Human CD11 C Antibody [clone: 3.9], monoclonal mouse IgG1. Validated for flow cytometry.
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Anti-Human IgD Heavy Chain Antibody, RayBright® Red 780 Human IgD Heavy Chain Antibody [clone: deltaTA4-1[dTA4-1]], monoclonal mouse IgG3 kappa. Validated for flow cytometry.
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Anti-Human IgM Antibody, RayBright® Red 780 Human IgM Antibody [clone: M-2E6], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
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Anti-Human CD29 Antibody, RayBright® Red 780 Human CD29 Antibody [clone: TS2/16.2.1 ], monoclonal mouse IgG1 kappa. Validated for flow cytometry. -
Anti-Human CD20 Antibody, RayBright® Red 780 Human CD20 Antibody [clone: C273 ], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
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Anti-Human CD36 Antibody, RayBright® Red 780 Human CD36 Antibody [clone: 8C9], monoclonal mouse IgM. Validated for flow cytometry.
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Anti-CD31 Antibody, RayBright® Red 780 Human / Mouse CD31 Antibody [clone: 2H8-f], monoclonal Armenian Hamster IgG. Validated for flow cytometry.
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Anti-Human CD13 Antibody, RayBright® Red 780 Human CD13 Antibody [clone: HBB3/153/63-g], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
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Anti-Human CD279 (PD1) Antibody, RayBright® Red 780 Human CD279 (PD1) Antibody [clone: 3B10], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
What customers are saying
“Kelly was helpful all along with the technique, sample preparation, and data analysis. Although Ray is a commercial company there was no tendency to hide information. The experience was just like collaborating with an academic research lab.”
Dhruba
NIH
“I spoke with Brianne on the phone, she was very helpful, knowledgeable, and quick in her response to my technical question. Probably one of the best tech support interactions I've had in biological sciences.”
James
Colorado State University
Featured Service
Flow Cytometry Reagents Services
Our experienced flow cytometry service team can help you evaluate a wide range of cellular processes, quantify cell populations, and even shed light on intracellular signaling events.
Basic Flow Cytometry Protocol
Cell Preparation
Gently harvest and wash cells, preferably with ice cold (4°C) reagents to preserve cell physiology.
Sample Blocking
Incubate suspended cells in a blocking buffer to prevent non-specific binding of primary antibodies
Antibody Incubation
Perform primary and secondary antibody incubations with dilutions based on cell concentration.
Data Acquisition
Run your sample through the flow cytometer and analyze your data.
Why use RayBiotech flow cytometry antibodies?
- High quality you can trust
- Cost effective so you can run more experiments
- Hard-to-find violet channel antibodies allowing you more flexibility
- Free trial sizes (5 µg) of any of our RayBright® antibodies so you can try before you buy
- Custom antibody production for you unique studies (learn more)
- Custom fluorophore conjugation with limitless possibilities
- Bulk discounts and long-term supply agreements so you always have what you need
Excitation and emission spectra of RayBright fluorochromes and equivalent dyes
| RayBiotech Superior Fluorochromes | Excitation Laser | Max Excitation/Emission | Replacing dyes of equivalent wavelengths |
|---|---|---|---|
| RayBright® UV 450 | UV 355 | 347/448 nm | Alexa Fluor 350 |
| RayBright® Violet 450 | Violet 407 | 403/454 nm | Pacific Blue, Alexa Fluor 450 |
| RayBright® Violet 500 | Violet 407 | 405/537 nm | Alexa Fluor 430, BV510 |
| RayBright® Violet 600 | Violet 407 | 407/600 nm | BD Brilliant Violet 605 |
| RayBright® Violet 655 | Violet 407 | 407/655 nm | BD Brilliant Violet 655 |
| RayBright® Blue 488 | Blue 488 | 491/518 nm | Alexa Fluor 488, FITC, BD BB515 |
| RayBright® Blue/Green 575 | Blue 488/Green 561 | 505/570, 553/570 nm | R-PE, Cy3 |
| RayBright® Green 594 | Green 561 | 594/614 nm | PE-Texas Red, PE-CF594, PE-eFluor610 |
| RayBright® Blue/Green 647 (PE-tandem dye) | Blue 488/Green 561 | 496/664, 565/664 nm | PE-Cy5 |
| RayBright® Blue/Green 780 (PE-tandem dye) | Blue 488/Green 561 | 496/780, 565/780 nm | PE-Cy7, PE-Alexa Fluor 750 |
| RayBright® Red 647 | Red 640 | 649/664 nm | APC, Alexa Fluor 647, Cy5 |
| RayBright® Red 700 | Red 640 | 685/710 nm | Alexa Fluor 700, APC-R700 |
| RayBright® Red 780 | Red 640 | 629/780 nm | Alexa Fluor 750, APC-eFluor 780 |
Frequently Asked Questions
Still have questions?
Still have questions?