RayBio® G-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 41 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Amphiregulin
| bFGF
| beta-NGF
| EGF
| EGFR
|
FGF-4
| FGF-6
| FGF-7 (KGF)
| GCSF
| GDNF
|
GM-CSF
| HB-EGF
| HGF
| IGFBP-1
| IGFBP-2
|
IGFBP-3
| IGFBP-4
| IGFBP-6
| IGF-1
| IGF-1 R
|
IGF-2
| M-CSF
| M-CSF R
| NT-3
| NT-4
|
PDGF R alpha
| PDGF R beta
| PDGF-AA
| PDGF-AB
| PDGF-BB
|
PLGF
| SCF
| SCF R (CD117/c-kit)
| TGF alpha
| TGF beta 1
|
TGF beta 2
| TGF beta 3
| VEGF-A
| VEGFR2
| VEGFR3
|
VEGF-D
| ||||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- Human Growth Factor Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Ding, C., Zou, Q., Wang, F. et al. Human amniotic mesenchymal stem cells improve ovarian function in natural aging through secreting hepatocyte growth factor and epidermal growth factor. Stem Cell Res Ther 9, 55 (2018). https://doi.org/10.1186/s13287-018-0781-9
Species:
Human
Sample Type:
Conditioned media (from hAMSCs)
Jeong, Yun-Mi, et al. "Ultraviolet B preconditioning enhances the hair growth-promoting effects of adipose-derived stem cells via generation of reactive oxygen species." Stem cells and development 22.1 (2012): 158-168.
Species:
Human
Sample Type:
Cell Lysate (adipose-derived stem cells UVB exposure)
Peloso A., Petrosyan A., Da Sacco S., et al. Renal Extracellular Matrix Scaffolds From Discarded Kidneys Maintain Glomerular Morphometry and Vascular Resilience and Retains Critical Growth Factors. Transplantation. 2015 Sep;99(9):1807-16. doi: 10.1097/TP.0000000000000811.
Species:
Human
Sample Type:
Tissue Lysate (Kidney and renal kidney extracellular decellularized kidney scaffolds)
Balasubramanian S., Venugopal P., Sundarraj S., et al. Comparison of chemokine and receptor gene expression between Wharton's jelly and bone marrow-derived mesenchymal stromal cells. Cytotherapy. 2012 Jan;14(1):26-33. doi: 10.3109/14653249.2011.605119.
Species:
Human
Sample Type:
Conditioned Media (Wharton's Jelly and BM derived MSCs)
Balasubramanian, Sudha, et al. "Higher propensity of Wharton's jelly derived mesenchymal stromal cells towards neuronal lineage in comparison to those derived from adipose and bone marrow." Cell Biology International 37.5 (2013): 507-515.
Species:
Human
Sample Type:
Conditioned Media (WJ, BM and AD derived MSC w)
Zhang M., Xu M., Zhou Z., Zhou J., Zhao Y., Wang Z., Lu M. The Differentiation of Human Adipose-Derived Stem Cells towards a Urothelium-Like Phenotype In Vitro and the Dynamic Temporal Changes of Related Cytokines by Both Paracrine and Autocrine Signal Regulation. PLOS One, Published: April 21, 2014, DOI: 10.1371/journal.pone.0095583 [Epub ahead of print]
Species:
Human
Sample Type:
Conditioned Media (Adipose-derived Stem Cells)
Petschnik, Anna E., et al. "A novel xenogeneic co-culture system to examine neuronal differentiation capability of various adult human stem cells." PloS one 6.9 (2011): e24944.
Species:
Rat
Sample Type:
Conditioned Media (co-culturing of SCs with rat brain biopsies)
Liu D., et al. SIX1 Promotes Tumor Lymphangiogenesis by Coordinating TGF? Signals That Increase Expression of VEGF-C . Cancer Res; Published OnlineFirst August 20, 2014; doi:10.1158/0008-5472.CAN-13-3598
Species:
Human
Sample Type:
Conditioned Media (Cervical Squamous Cell Carcinoma cells)
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