RayBio® C-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.
As low as $440.00
In stock
Only %1 left
Catalog #:
AAH-GF-1
-
+

Product Description

Specifications

Size 2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species Human
Quantitative/Semi-Quantitative Semi-Quantitative
Number of Targets Detected 41
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support Membrane
Method Of Detection Chemiluminescence
Design Principle Sandwich-based
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C

Risk-Free Guarantee

We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

Learn More
Amazon Gift Cards!

$5 Amazon gift card in every kit box purchased.

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Amphiregulin
bFGF
beta-NGF
EGF
EGFR
FGF-4
FGF-6
FGF-7
(KGF)
GCSF
GDNF
GM-CSF
HB-EGF
HGF
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
M-CSF
M-CSF R
NT-3
NT-4
PDGF R alpha
PDGF R beta
PDGF-AA
PDGF-AB
PDGF-BB
PLGF
SCF
SCF R
(CD117/c-kit)
TGF alpha
TGF beta 1
TGF beta 2
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Growth Factor Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuantâ„¢ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Lee E., Hwang I., Lee J., et al. Hepatocyte Growth Factor Improves the Therapeutic Efficacy of Human Bone Marrow Mesenchymal Stem Cells via RAD51. Mol Ther. 2018 Mar 7;26(3):845-859. doi: 10.1016/j.ymthe.2017.12.015.
Species:
Human
Sample Type:
Conditioned Media (Human Mesenchymal stem cells treated with HGF and other)
Chou Y., Young T., Lou P. Acta Biomater. Effects of biomaterial-derived fibroblast conditioned medium on the ?-amylase expression of parotid gland acinar cells. 2015 Nov;27:214-23. doi: 10.1016/j.actbio.2015.08.050.
Species:
Human
Sample Type:
Conditioned Media (Fibroblasts cells on various biomaterials)
Pickard A, McDade SS, McFarland M, McCluggage WG, Wheeler CM, McCance DJ (2015) HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion in Organotypic Cultures. PLoS Pathog 11(6): e1004988. doi:10.1371/journal.ppat.1004988
Species:
Human
Sample Type:
Conditioned Media (Conditioned form E6/7 Human foreskin keratinocytes)
Chan J., Tan M., Sng M., et al. Cancer-associated fibroblasts enact field cancerization by promoting extratumoral oxidative stress. Cell Death Dis 8 (1), e2562. 2017 Jan 19.
Species:
Human
Sample Type:
Conditioned Media (Cancer associated fibroblast cells in squamous cell carcinoma)
Kim E. Choi J., Kim J., et al. Injectable and Thermosensitive Soluble Extracellular Matrix and Methylcellulose Hydrogels for Stem Cell Delivery in Skin Wounds. Biomacromolecules. 2016 Jan 11;17(1):4-11. doi: 10.1021/acs.biomac.5b01566
Species:
Human
Sample Type:
Conditioned Media (Adipose tissue soluble extracellular matrix fluid)
Gaceb, Abderahim, et al. "Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB." Journal of Cerebral Blood Flow & Metabolism 38.1 (2018): 45-57.
Species:
Human
Sample Type:
Conditioned Media (human pericytes)
?engül B, Dursun E, Verkhratsky A, Gezen-Ak D. Overexpression of ?-Synuclein Reorganises Growth Factor Profile of Human Astrocytes. Mol Neurobiol. 2021 Jan;58(1):184-203. doi: 10.1007/s12035-020-02114-x. Epub 2020 Sep 10. PMID: 32914394.
Species:
Human
Sample Type:
Cell lysate (bFGF, ?NGF, EGF, EGFR, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-I sR, IGF-2, M-CSF, M-CSFR, NT-3, NT-4, PDGF AA, PDGF AB, PDGF BB, PDGFRa, PDGF Rb, PIGF, SCF, SCFR, TGF-?,
Gimenez-Bastida JA, Gonzalez-Sarrias A., Larrosa M., et al. Ellagitannin metabolites, urolithin A glucuronide and its aglycone urolithin A, ameliorate TNF-?-induced inflammation and associated molecular markers in human aortic endothelial cells.Mol Nutr Food Res. 2012 May;56(5):784-96. doi: 10.1002/mnfr.201100677
Species:
Human
Sample Type:
Conditioned Media (TNF treated human aortic endothelial cells (HAEC))
Christov C, Cretien F, Abou-Kalil R, Bassez G, Vallet G, et al. Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners. Mol Biol Cell. 2007;18:1397-1409.
Species:
Human
Sample Type:
Conditioned Media (Human umbilical vein endothelial (HUVEC) cell-cultured medium)
Lai, Kuo-Pao, et al. "Targeting stromal androgen receptor suppresses prolactin-driven benign prostatic hyperplasia (BPH)." Molecular endocrinology 27.10 (2013): 1617-1631.
Species:
Human
Sample Type:
Conditioned Media (BPH-1-PRLR or BPH-1-PRLR with 100 ng/mL human PRL (hPRL) (Benign Prostatic Hyperplasia (BPH))
  1. Great product
    Rating
    100%
    We have utilized this kit to evaluate the growth factor components from the human adipose derived mesenchymal cell secretome (https://www.sciencedirect.com/science/article/pii/S2452199X21005508). The assay was sensitive and easy to perform and the results were reliable. We really like this product.

    Review by

    from University of Nebraska Medical Center,

    on

  2. Great product
    Rating
    80%
    A high throughput assay can give you 42 GFs levels in your testest sample within 48 hrs. Easy to do and read the dots.

    Review by

    from Cleveland Clinic,

    on

  3. Great product
    Rating
    100%
    We have purchased several kits of the AAH-GF-1-8. It's is sensitive and reliable. It's easy to handle and perform the assay. We are really glad to find this product and use it in our projects.

    Review by

    from University of Nebraska Medical Center,

    on

Write Your Own Review
Your Rating
Submit a review for this product on Biocompare.com to earn an entry into a monthly raffle for one of five $100 Amazon gift cards!

Ask a Question

Display name:
Your location:*
This field is for validation purposes and should be left unchanged.