Product Description
Specifications
Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
---|---|
Species | Human |
Quantitative/Semi-Quantitative | Semi-Quantitative |
Number of Targets Detected | 41 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Membrane |
Method Of Detection | Chemiluminescence |
Design Principle | Sandwich-based |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | -20°C |
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Scroll over each target protein for more information | ||||
---|---|---|---|---|
Amphiregulin
|
bFGF
|
beta-NGF
|
EGF
|
EGFR
|
FGF-4
|
FGF-6
|
FGF-7
(KGF)
|
GCSF
|
GDNF
|
GM-CSF
|
HB-EGF
|
HGF
|
IGFBP-1
|
IGFBP-2
|
IGFBP-3
|
IGFBP-4
|
IGFBP-6
|
IGF-1
|
IGF-1 R
|
IGF-2
|
M-CSF
|
M-CSF R
|
NT-3
|
NT-4
|
PDGF R alpha
|
PDGF R beta
|
PDGF-AA
|
PDGF-AB
|
PDGF-BB
|
PLGF
|
SCF
|
SCF R
(CD117/c-kit)
|
TGF alpha
|
TGF beta 1
|
TGF beta 2
|
TGF beta 3
|
VEGF-A
|
VEGFR2
|
VEGFR3
|
VEGF-D
|
Application Notes
- Human Growth Factor Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
Lee E., Hwang I., Lee J., et al. Hepatocyte Growth Factor Improves the Therapeutic Efficacy of Human Bone Marrow Mesenchymal Stem Cells via RAD51. Mol Ther. 2018 Mar 7;26(3):845-859. doi: 10.1016/j.ymthe.2017.12.015.
Chou Y., Young T., Lou P. Acta Biomater. Effects of biomaterial-derived fibroblast conditioned medium on the ?-amylase expression of parotid gland acinar cells. 2015 Nov;27:214-23. doi: 10.1016/j.actbio.2015.08.050.
Pickard A, McDade SS, McFarland M, McCluggage WG, Wheeler CM, McCance DJ (2015) HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion in Organotypic Cultures. PLoS Pathog 11(6): e1004988. doi:10.1371/journal.ppat.1004988
Chan J., Tan M., Sng M., et al. Cancer-associated fibroblasts enact field cancerization by promoting extratumoral oxidative stress. Cell Death Dis 8 (1), e2562. 2017 Jan 19.
Kim E. Choi J., Kim J., et al. Injectable and Thermosensitive Soluble Extracellular Matrix and Methylcellulose Hydrogels for Stem Cell Delivery in Skin Wounds. Biomacromolecules. 2016 Jan 11;17(1):4-11. doi: 10.1021/acs.biomac.5b01566
Gaceb, Abderahim, et al. "Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB." Journal of Cerebral Blood Flow & Metabolism 38.1 (2018): 45-57.
?engül B, Dursun E, Verkhratsky A, Gezen-Ak D. Overexpression of ?-Synuclein Reorganises Growth Factor Profile of Human Astrocytes. Mol Neurobiol. 2021 Jan;58(1):184-203. doi: 10.1007/s12035-020-02114-x. Epub 2020 Sep 10. PMID: 32914394.
Gimenez-Bastida JA, Gonzalez-Sarrias A., Larrosa M., et al. Ellagitannin metabolites, urolithin A glucuronide and its aglycone urolithin A, ameliorate TNF-?-induced inflammation and associated molecular markers in human aortic endothelial cells.Mol Nutr Food Res. 2012 May;56(5):784-96. doi: 10.1002/mnfr.201100677
Christov C, Cretien F, Abou-Kalil R, Bassez G, Vallet G, et al. Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners. Mol Biol Cell. 2007;18:1397-1409.
Lai, Kuo-Pao, et al. "Targeting stromal androgen receptor suppresses prolactin-driven benign prostatic hyperplasia (BPH)." Molecular endocrinology 27.10 (2013): 1617-1631.
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Great productWe have utilized this kit to evaluate the growth factor components from the human adipose derived mesenchymal cell secretome (https://www.sciencedirect.com/science/article/pii/S2452199X21005508). The assay was sensitive and easy to perform and the results were reliable. We really like this product.
from University of Nebraska Medical Center,
on
Great productA high throughput assay can give you 42 GFs levels in your testest sample within 48 hrs. Easy to do and read the dots.from Cleveland Clinic,
on
Great productWe have purchased several kits of the AAH-GF-1-8. It's is sensitive and reliable. It's easy to handle and perform the assay. We are really glad to find this product and use it in our projects.from University of Nebraska Medical Center,
on
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