Quantibody® Human Growth Factor Array 1 Kit. Detects 40 Human Growth Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 40 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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$5 Amazon gift card in every kit box purchased.
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Amphiregulin
| BDNF
| bFGF
| BMP-4
| BMP-5
|
BMP-7
| beta-NGF
| EGF
| EGFR
| EG-VEGF (PK1)
|
FGF-4
| FGF-7 (KGF)
| GDF-15
| GDNF
| Growth Hormone
|
HB-EGF
| HGF
| IGFBP-1
| IGFBP-2
| IGFBP-3
|
IGFBP-4
| IGFBP-6
| IGF-1
| Insulin
| M-CSF R
|
NGFR (TNFRSF16)
| NT-3
| NT-4
| Osteoprotegerin (TNFRSF11B)
| PDGF-AA
|
PLGF
| SCF
| SCF R (CD117/c-kit)
| TGF alpha
| TGF beta 1
|
TGF beta 3
| VEGF-A
| VEGFR2
| VEGFR3
| VEGF-D
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Growth Factor Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Skardal A., Devarasetty M., Kang H., et al. A hydrogel bioink toolkit for mimicking native tissue biochemical and mechanical properties in bioprinted tissue constructs. Acta Biomater. 2015 Oct;25:24-34. doi: 10.1016/j.actbio.2015.07.030.
Species:
Human
Sample Type:
Tissue Lysate (Extracellular matrix lysates from liver, cardiac and skeletal muscle)
Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.
Species:
Human
Sample Type:
Vitreous Fluid (Vitreous sample from retinopathy patients)
Skardal A., Mack D., Kapetanovic E., et al. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Translational Medicine. 2012;1(11):792-802. doi: 10.5966/sctm.2012-0088.
Species:
Human
Sample Type:
Conditioned Media (mniotic fluid derived stem cells, AFS, and MSCs)
Velez G., Roybal C., Colgan D., et al. Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease. JAMA Ophthalmol. 2016 Apr;134(4):444-8. doi: 10.1001/jamaophthalmol.2015.5934.
Species:
Human
Sample Type:
Vitreous Fluid (Human Vitreous Fluid samples)
Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.
Species:
Human
Sample Type:
Conditioned Media (Embryonic stem cell derived astrocytes in immunodeficient mice)
Yang B., Deng Q., Zhang W., et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep. 2016 Jan 13;6:19272. doi: 10.1038/srep19272.
Species:
Human
Sample Type:
Plasma (Polycyclic aromatic hydrocarbon exposed patients)
Reategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094
Species:
Human
Sample Type:
Conditioned Media (Neutrophil swarming experiments)
Leung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327
Species:
Human
Sample Type:
Conditioned Media (A375 ERK2 treated cells with or without ddoxycycline)
Panchaprateep, R., W. Korkij, and P. Asawanonda. "Brain?derived nerve factor and neurotrophins in androgenetic alopecia." British Journal of Dermatology 165.5 (2011): 997-1002.
Species:
Human
Sample Type:
Conditioned Media (Dermal papilla (DP) cells)
Ciavarella S., Caselli A., Tamma A., et al. A peculiar molecular profile of umbilical cord-mesenchymal stromal cells drives their inhibitory effects on multiple myeloma cell growth and tumor progression. Stem Cells Dev. 2015 Jun 15;24(12):1457-70. doi: 10.1089/scd.2014.0254.
Species:
Human
Sample Type:
Conditioned Media (MSC, umbilical and bone marrow stem cell cultures)
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Human factor array Q1Easy to use with reproducible results. Plan to use it again.
from USC,
on
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Human Growth Factor Array Q1Very easy to use. Sample processing is straight forward and allows multiple sample processing at the same time. I'd recommend this product.
from Gateway Institute for Brain Research,
on
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Great product.
from Surgenex LLC,
on
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Great productI really like the Quantibody Arrays. The data is consistent and reproducible. Important to strictly follow protocol, but very straightforward. Plan on using again.
from University of California Davis,
on
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