Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
Human Growth Factor Array Q1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual
Other Materials Required
Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Skardal A., Devarasetty M., Kang H., et al. A hydrogel bioink toolkit for mimicking native tissue biochemical and mechanical properties in bioprinted tissue constructs. Acta Biomater. 2015 Oct;25:24-34. doi: 10.1016/j.actbio.2015.07.030.
Species:
Human
Sample Type:
Tissue Lysate (Extracellular matrix lysates from liver, cardiac and skeletal muscle)
Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.
Species:
Human
Sample Type:
Vitreous Fluid (Vitreous sample from retinopathy patients)
Skardal A., Mack D., Kapetanovic E., et al. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Translational Medicine. 2012;1(11):792-802. doi: 10.5966/sctm.2012-0088.
Species:
Human
Sample Type:
Conditioned Media (mniotic fluid derived stem cells, AFS, and MSCs)
Velez G., Roybal C., Colgan D., et al. Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease. JAMA Ophthalmol. 2016 Apr;134(4):444-8. doi: 10.1001/jamaophthalmol.2015.5934.
Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.
Species:
Human
Sample Type:
Conditioned Media (Embryonic stem cell derived astrocytes in immunodeficient mice)
Yang B., Deng Q., Zhang W., et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep. 2016 Jan 13;6:19272. doi: 10.1038/srep19272.
Reategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094
Species:
Human
Sample Type:
Conditioned Media (Neutrophil swarming experiments)
Leung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327
Species:
Human
Sample Type:
Conditioned Media (A375 ERK2 treated cells with or without ddoxycycline)
Panchaprateep, R., W. Korkij, and P. Asawanonda. "Brain?derived nerve factor and neurotrophins in androgenetic alopecia." British Journal of Dermatology 165.5 (2011): 997-1002.
Ciavarella S., Caselli A., Tamma A., et al. A peculiar molecular profile of umbilical cord-mesenchymal stromal cells drives their inhibitory effects on multiple myeloma cell growth and tumor progression. Stem Cells Dev. 2015 Jun 15;24(12):1457-70. doi: 10.1089/scd.2014.0254.
Species:
Human
Sample Type:
Conditioned Media (MSC, umbilical and bone marrow stem cell cultures)
Easy to use with reproducible results. Plan to use it again.
Review byDP,
fromUSC,
on
Human Growth Factor Array Q1
Rating
80%
Very easy to use. Sample processing is straight forward and allows multiple sample processing at the same time. I'd recommend this product.
Review bySandhya Sharma,
fromGateway Institute for Brain Research,
on
Great product
Rating
100%
.
Review byMilla Zakharova,
fromSurgenex LLC,
on
Great product
Rating
100%
I really like the Quantibody Arrays. The data is consistent and reproducible. Important to strictly follow protocol, but very straightforward. Plan on using again.
