Quantibody® Mouse Cytokine Array 4000 Kit. A combination of Mouse Cytokine Array Q4, Mouse Cytokine Array Q5, Mouse Cytokine Array Q6, Mouse Cytokine Array Q7, and Mouse Cytokine Array Q8. Detects 200 Mouse Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
---|---|
Species | Mouse |
Quantitative/Semi-Quantitative | Quantitative |
Number of Targets Detected | 200 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
Solid Support | Glass Slide |
Method Of Detection | Fluorescence Laser Scanner |
Design Principle | Sandwich-based |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | -20°C |
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Scroll over each target protein for more information | ||||
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4-1BB
(TNFRSF9/CD137)
|
6Ckine
(CCL21)
|
Ace
|
Activin A
|
ADAMTS-1
(METH1)
|
Adiponectin
(ACRP30)
|
ALK-1
|
Amphiregulin
|
ANGPTL1
|
ANGPTL3
|
Artemin
|
Axl
|
BAFF R
|
Betacellulin
(BTC)
|
bFGF
|
BLC
(CXCL13)
|
C5a
|
Cardiotrophin-1
(CT-1)
|
CCL28
(MEC)
|
CCL6
|
CD27
(TNFRSF7)
|
CD27 Ligand
(TNFSF7)
|
CD30
(TNFRSF8)
|
CD30 Ligand
(TNFSF8)
|
CD32a
|
CD36
(SR-B3)
|
CD40
(TNFRSF5)
|
CD40 Ligand
(TNFSF5)
|
CD48
(SLAMF2)
|
CD6
|
CD80
(B7-1)
|
Chemerin
|
Chordin
|
Clusterin
|
CRP
(C-Reactive Protein)
|
CTLA-4
(CD152)
|
CXCL16
|
Cystatin C
|
DAN
|
Decorin
|
DKK-1
|
DLL4
|
Dtk
|
E-Cadherin
|
EDAR
|
EGF
|
Endocan
|
Endoglin
(CD105)
|
Eotaxin-1
(CCL11)
|
Eotaxin-2
(MPIF-2/CCL24)
|
Epigen
|
Epiregulin
|
E-Selectin
|
Fas
(TNFRSF6/Apo-1)
|
Fas Ligand
(TNFSF6)
|
Fetuin A
|
Flt-3 Ligand
|
Fractalkine
(CX3CL1)
|
Galectin-1
|
Galectin-3
|
Galectin-7
|
Gas 1
|
Gas 6
|
GCSF
|
GITR
(TNFRSF18)
|
GITR Ligand
(TNFSF18)
|
GM-CSF
|
gp130
|
Granzyme B
|
Gremlin-1
|
H60
|
HAI-1
|
HGF
|
HGFR
|
I-309
(TCA-3/CCL1)
|
ICAM-1
(CD54)
|
IFN-gamma
|
IFN-gamma R1
|
IGF-1
|
IGFBP-2
|
IGFBP-3
|
IGFBP-5
|
IGFBP-6
|
IL-1 alpha
(IL-1 F1)
|
IL-1 beta
(IL-1 F2)
|
IL-1 R4
(ST2)
|
IL-1 ra
(IL-1 F3)
|
IL-10
|
IL-12 p40
|
IL-12 p70
|
IL-13
|
IL-15
|
IL-17 RB
|
IL-17A
|
IL-17B
|
IL-17E
(IL-25)
|
IL-17F
|
IL-2
|
IL-2 R alpha
|
IL-20
|
IL-21
|
IL-22
|
IL-23 p19
|
IL-28A
(IFN-lambda 2)
|
IL-3
|
IL-3 R beta
|
IL-33
(IL-1 F11)
|
IL-4
|
IL-5
|
IL-6
|
IL-7
|
IL-7 R alpha
|
IL-9
|
I-TAC
(CXCL11)
|
JAM-A
(CD321/F11R)
|
KC
(CXCL1)
|
Kremen-1
|
Leptin
|
Leptin R
|
Limitin
|
Lipocalin-2
(NGAL)
|
LIX
|
LOX-1
|
L-Selectin
(CD62L)
|
Lungkine
(CXCL15)
|
Lymphotactin
(XCL1)
|
MAdCAM-1
|
Marapsin
|
MBL-2
|
MCP-1
(CCL2)
|
MCP-5
|
M-CSF
|
MDC
(CCL22)
|
Meteorin
|
MFG-E8
|
MIG
(CXCL9)
|
MIP-1 alpha
(CCL3)
|
MIP-1 beta
(CCL4)
|
MIP-1 gamma
|
MIP-2
|
MIP-3 alpha
(CCL20)
|
MIP-3 beta
(CCL19)
|
MMP-10
|
MMP-2
|
MMP-3
|
Neprilysin
|
Nope
|
NOV
(CCN3)
|
Osteoactivin
(GPNMB)
|
Osteopontin
(SPP1)
|
Osteoprotegerin
(TNFRSF11B)
|
OX40 Ligand
|
P-Cadherin
|
PDGF-AA
|
Pentraxin-3
(TSG-14)
|
Periostin
|
Persephin
|
Platelet Factor 4
(CXCL4)
|
PlGF-2
|
Progranulin
|
Prolactin
|
Pro-MMP-9
|
Prostasin
|
P-Selectin
|
RAGE
|
RANTES
(CCL5)
|
Renin 1
|
Resistin
|
SCF
|
SDF-1 alpha
(CXCL12 alpha)
|
sFRP-3
|
SLAM
(CD150)
|
Sonic Hedgehog N-Terminal
(Shh-N)
|
TACI
(TNFRSF13B)
|
TARC
(CCL17)
|
TCK-1
(CXCL7)
|
TECK
(CCL25)
|
Testican 3
|
TGF beta 1
|
Thrombopoietin
(TPO)
|
TIM-1
(KIM-1)
|
TNF alpha
|
TNF RI
(TNFRSF1A)
|
TNF RII
(TNFRSF1B)
|
TRAIL
(TNFSF10)
|
Trance
(TNFSF11)
|
TREM-1
|
TREML1
(TLT-1)
|
TROY
(TNFRSF19)
|
Tryptase epsilon
|
TSLP
|
TWEAK
(TNFSF12)
|
TWEAK R
(TNFRSF12)
|
VCAM-1
(CD106)
|
VEGF-A
|
VEGF-B
|
VEGF-D
|
VEGFR1
|
VEGFR2
|
VEGFR3
|
Species Detected
Mouse
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q4000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Yang, Y., He, Z., Xing, Z. et al. Influenza vaccination in early Alzheimer's disease rescues amyloidosis and ameliorates cognitive deficits in APP/PS1 mice by inhibiting regulatory T cells. J Neuroinflammation 17, 65 (2020). https://doi.org/10.1186/s12974-020-01741-4
Species:
Mouse
Sample Type:
Serum, Tissue Lysate (brain lysate)
Mao Y., Yen H., Sun Y., Lv Z., Huang R. Development of non-overlapping multiplex antibody arrays for the quantitative measurement of 400 human and 200 mouse proteins in parallel (TECH1P.849). The Journal of ImmunologyMay 1, 2014vol. 192 no. 1 Supplement 69.17
Species:
Mouse
Sample Type:
Plasma
Mao Y., Yen H., Sun Y., Lv Z., Huang R. Development of non-overlapping multiplex antibody arrays for the quantitative measurement of 400 human and 200 mouse proteins in parallel (TECH1P.849). The Journal of ImmunologyMay 1, 2014vol. 192 no. 1 Supplement 69.17
Species:
Mouse
Sample Type:
Serum
Lai H, Zhao X, Qin Y, Ding Y, Chen R, Li G, Labrie M, Ding Z, Zhou J, Hu J, Ma D, Fang Y, Gao Q. FAK-ERK activation in cell/matrix adhesion induced by the loss of apolipoprotein E stimulates the malignant progression of ovarian cancer. J Exp Clin Cancer Res. 2018 Feb 20;37(1):32. doi: 10.1186/s13046-018-0696-4. Erratum in: J Exp Clin Cancer Res. 2019 Oct 15;38(1):415. PMID: 29458390; PMCID: PMC5819228.
Species:
Mouse
Sample Type:
Conditioned Media
Yariswamy M., Yoshida T., Valente A., et al. Cardiac-restricted Overexpression of TRAF3 Interacting Protein 2 (TRAF3IP2) Results in Spontaneous Development of Myocardial Hypertrophy, Fibrosis, and Dysfunction. J Biol Chem. 2016 Sep 9;291(37):19425-36. doi: 10.1074/jbc.M116.724138
Species:
Mouse
Sample Type:
Tissue Lysate (Left Ventricular lysates in a cardiac stress model)
Erikson J., Valente A., Mummidi S., et al. Targeting TRAF3IP2 by genetic and interventional approaches inhibits ischemia/reperfusion-induced myocardial injury and adverse remodeling. J Biol Chem. 2017 Feb 10;292(6):2345-2358. doi: 10.1074/jbc.M116.764522.
Species:
Mouse
Sample Type:
Tissue Lysate (Left Ventricular lysates in a cardiac stress model)
Kurtz S., Elkins K. Correlates of vaccine-induced protection against TB immune revealed in comparative analyses of lymphocyte populations. Clinical and Vaccine Immunology, Accepted manuscript posted online 12 August 2015, doi: 10.1128/CVI.00301-15
Species:
Mouse
Sample Type:
Conditioned Media (CO coluture of PBMC and splenocytes)
Wang Y, Tan J, Hu P, Pei Q, Wen Y, Ma W, Shi K, Li Z, Li H, Cheng F, Gu X, Yao X, Man Y, Zhao R, Feng S, Ding X, Yang T. Traditional Chinese medicine compound, Bu Sheng Hui Yang Fang, promotes the proliferation of lymphocytes in the immunosuppressed mice potentially by upregulating IL-4 signaling. Biomed Pharmacother. 2020 Dec 16;134:111107. doi: 10.1016/j.biopha.2020.111107. Epub ahead of print. PMID: 33341059.
Species:
Mice
Sample Type:
Serum
Schnepp P., Lee D., Guldner I., et al. GAD1 Upregulation Programs Aggressive Features of Cancer Cell Metabolism in the Brain Metastatic Microenvironment. Cancer Res. 2017 Apr 11. pii: canres.2289.2016. doi: 10.1158/0008-5472.CAN-16-2289.
Species:
Mouse
Sample Type:
Conditioned Media (Cancer associated fibroblasts and tumor cells in a transwell setup)
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