RayBio® Mouse GM-CSF ELISA Kit for cell culture supernatants, plasma, and serum samples.

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As low as $420.00
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Catalog #:
ELM-GMCSF
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Mouse
Accession Number P01587
Gene Id 12981
Gene Symbols CSF2|CSFGM
Protein Name / Synonyms Granulocyte-macrophage colony-stimulating factor (GM-CSF) (Colony-stimulating factor) (CSF)
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Sensitivity 6 pg/ml

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Detection Range 6 pg/ml - 2000 pg/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Zhang H., Ren Y., Tang X., et al. Vascular Normalization Induced by Sinomenine Hydrochloride Results in Suppressed Mammary Tumor Growth and Metastasis. Sci Rep. 2015 Mar 9;5:8888. doi: 10.1038/srep08888.
Species:
Mouse
Sample Type:
Tissue Lysate (Mammary Tumor lysate)
Shukla, Mamta, et al. "Carryover of cigarette smoke effects on hematopoietic cytokines to F 1 mouse litters." Molecular immunology 48.15 (2011): 1809-1817.
Species:
Mouse
Sample Type:
Serum
Shukla, Mamta, et al. "Carryover of cigarette smoke effects on hematopoietic cytokines to F 1 mouse litters." Molecular immunology 48.15 (2011): 1809-1817.
Species:
Mouse
Sample Type:
BAL Fluid
Shukla, Mamta, et al. "Carryover of cigarette smoke effects on hematopoietic cytokines to F 1 mouse litters." Molecular immunology 48.15 (2011): 1809-1817.
Species:
Mouse
Sample Type:
Amniotic fluid
Shukla, Mamta, et al. "Carryover of cigarette smoke effects on hematopoietic cytokines to F 1 mouse litters." Molecular immunology 48.15 (2011): 1809-1817.
Species:
Mouse
Sample Type:
Bone Marrow Aspirate
Li, R., Qi, Y., Jiang, M., Zhang, T., Wang, H., Wang, L., & Han, M. (2019). Primary tumor?secreted VEGF induces vascular hyperpermeability in premetastatic lung via the occludin phosphorylation/ubiquitination pathway. Molecular Carcinogenesis. doi:10.1002/mc.23120 
Species:
Mouse
Sample Type:
Serum, Conditioned Media
Mishra S., et al. Semiquinone Glucoside Derivative Provides Protection Against c-Radiation by Modulation of Immune Response in Murine Model. Environmental Toxicology, Epub ahead of print Oct 2014.DOI: 10.1002/tox.22061
Species:
Mouse
Sample Type:
Serum (Semiquinone Glucoside derivative in irradiated mice)
Ye P, Chen W, Wu J, et al. GM-CSF contributes to aortic aneurysms resulting from SMAD3 deficiency. J. Clin. Invest. 2013 May 1;123(5):2317-2331.
Species:
Mouse
Sample Type:
Conditioned Media (CD4+ T cells)
  1. Great product
    Rating
    60%
    Mouse serum samples unexpectedly below signal threshold. Standards and reference samples worked appropriately, so a certain amount of troubleshooting may be required with different mouse models. Overall great kit, however.

    Review by

    from Albert Einstein College of Medicine,

    on

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