RayBio® Mouse IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Mouse |
| Accession Number | P08505 |
| Gene Id | 16193 |
| Gene Symbols | IL6|IL-6 |
| Protein Name / Synonyms | Interleukin-6 (IL-6) (B-cell hybridoma growth factor) (Interleukin HP-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 600 pg/ml |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Mouse IL-6 ELISA KitRecently used the IL-6 ELISA kit for quantifying interleukin-6 (IL-6) levels in cachexia mouse plasma samples. The kit came with all necessary reagents, including pre-coated 96-well plates, standards, detection antibodies, and substrates, which minimized preparation time and potential errors. The IL-6 ELISA kit protocol was straightforward, and all the assay incubations were at RT. The dilutions (2-fold) worked good as suggested in the protocol.
Will order this kit for our experiments further.
from University of Oklahoma health sciences center,
on
Finally! A tissue homogenate-compatible IL-6 ELISA!We've been looking for an ELISA kit that detects IL-6 in tissue lysates for ages. We used this kit with mouse brain tissue homogenized in a lysis buffer containing 0.25% SDS and diluted the samples 1:5 as recommended by the manufacturer protocol and got beautiful results.from University of Illinois at Chicago,
on
Great productI recently had the opportunity to use the IL-6 ELISA kit for quantifying interleukin-6 (IL-6) levels in mouse skin tissue samples.
From the outset, the IL-6 ELISA kit was straightforward and user-friendly. The kit came with all necessary reagents, including pre-coated 96-well plates, standards, detection antibodies, and substrates, which minimized preparation time and potential errors.from Western University of Health Science,
on
Excellent Eisa kitThis IL-6 ELISA Kit is highly recommended for measuring IL-6 in inflammatory research studies due to its exceptional sensitivity, reproducibility, specificity, and dynamic range. I obtained good results when using it for mouse skin.from Western University of Health Science,
on
Great productVery simple kit to use, tricky with mouse plasma samples thoughfrom BCM,
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Great productElisa Mouse IL-6, I tested some plasma samples, and I was not able to detect any IL-6 even after incubating overnight at 4Cfrom University of Michigan,
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Great productI used this kit to quantify IL-6 levels in whole brain lysates. Protocol is clear and easy, the assay works wellfrom Cold Spring Harbor Laboratory,
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Great productI analyzed mouse plasma and brain tissue with this ELISA. The directions were clear and easy to follow. No problems were noted. Would use again!from University of Mary Washington,
on
Great productI have used this Elisa kit, its quite affordable considering the amount of time saved and data generated from it. I have even compared IL-6 ELISA data to IL-6 luminex data (from the same samples) and they are relatively similar.from NanoBST,
on
Great productI used this kit with LPS-inflammed RAW264.7 cells supernatants. It was very sensitive! The instructions were clear and detailed and the kit well assembled. I also liked that you can use just half a plate and keep the other strips for another time. I would strongly recommend.from University of Arkansas,
on
Great productI used this ELISA kit to measure IL-6 in mouse serum. While this was not the easiest ELISA to perform, the instructions were simple enough to follow.from Emory University,
on
Great productProtocol was very easy to follow and very reliable. Cost effective kit!from NanoBST,
on
Great productI tested mouse serum. I did not measure any signals.from FDA,
on
Great productThe kit was packaged in an organized and clear manner, and it was easy to walk through each step of the procedure. I used the mouse IL-6, VEGF, and TNF-alpha kits for plasma as well as homogenized muscle samples. Each worked well, and I would be happy to use them again.from Houston Methodist Research Institute,
on
Great productI used this kit to analyze mouse serum samples and it did exactly what it is supposed to do. I highly recommend this product!from BTS Research,
on
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