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    Mouse Adiponectin ELISA Kit

    Rating:
    85 % of 100
    4  Reviews
    4 Citations

    RayBio® Mouse Adiponectin (ACRP30) ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

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    As low as $420.00
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    Catalog #:
    ELM-Adiponectin
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Mouse
    Accession Number Q60994
    Gene Id 11450
    Gene Symbols ADIPOQ|ACRP30|ACDC|APM1
    Protein Name / Synonyms Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipocyte-specific protein AdipoQ)
    Quantitative/Semi-Quantitative Quantitative
    Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS-1, Angiopoietin-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17 RB, IL-17B, IL-22, MIP-1 beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGFbeta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2.
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Research Area Cardiovascular Disease
    Sensitivity 49 pg/ml

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    Detection Range 49 pg/ml - 12,000 pg/ml
    Recommended Dilution (Serum/Plasma) 20,000 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage ≤-20°C

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Citations

    Allen, London, et al. "Effects of delta-tocotrienol on obesity-related adipocyte hypertrophy, inflammation and hepatic steatosis in high-fat-fed mice." The Journal of nutritional biochemistry 48 (2017): 128-137.
    Species:
    Mouse
    Sample Type:
    Serum (adiponectin serum)
    View Publication
    Wang, Xin-An, et al. "CARD3 deficiency exacerbates diet-induced obesity, hepatosteatosis, and insulin resistance in male mice." Endocrinology 154.2 (2013): 685-697.
    Species:
    Mouse
    Sample Type:
    Serum (CARD3-knockout (KO) and wild-type (WT) mice fed a high-fat diet (HFD) to study obesity/insulin resistance)
    View Publication
    Wang, Xin-An, et al. "Interferon regulatory factor 7 deficiency prevents diet-induced obesity and insulin resistance." American Journal of Physiology-Endocrinology and Metabolism 305.4 (2013): E485-E495.
    Species:
    Mouse
    Sample Type:
    Serum (C57BL/6 and IRF7 knockout mice - diet induced insulin resistance obesity model)
    View Publication
    Eo H., Jeon Yj., Lee M., et al. Brown Alga Ecklonia cava Polyphenol Extract Ameliorates Hepatic Lipogenesis, Oxidative Stress, and Inflammation by Activation of AMPK and SIRT1 in High-Fat Diet-Induced Obese Mice. J Agric Food Chem. 2014 Dec 22.
    Species:
    Mouse
    Sample Type:
    Plasma (High-fat diet mice)
    View Publication

    Reviews

    Write a customer review
    1. Elisa Kit
      Rating
      80%
      The kit protocol was extremely straightforward and my lab was able to gather result quickly.

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      on

    2. Great product
      Rating
      100%
      This worked great for us - great instructions and simple.

      Review by

      from LSU Health Sciences Center,

      on

    3. Great product
      Rating
      80%
      Great sensitivity and precision on our mouse serum samples! Easy to use kit with clear instructions.

      Review by

      from Albert Einstein College of Medicine,

      on

    4. Great product
      Rating
      80%
      The kit works as intended. I like the fact that the recommended dilution is 20,000 so it allowed me to only waste a couple of microliters of our precious plasma samples.

      Review by

      from Wake Forest University,

      on

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