Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Mouse
Accession Number	Q00731
Gene Id	22339
Gene Symbols	VEGFA\|VEGF
Protein Name / Synonyms	Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Research Area	Angiogenesis
Sensitivity	2 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	2 pg/ml - 1000 pg/ml
Recommended Dilution (Serum/Plasma)	3 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELM-VEGF.pdf)

Datasheet (ELM-VEGF-CL.pdf)

SDS (ELM-VEGF.pdf)

SDS (ELM-VEGF-CL.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Zhang J., Wu Y., Chen A., Zhao Q. Mesenchymal Stem Cells Promote Cardiac Muscle Repair via Enhanced Neovascularization. Cell Physiol Biochem. 2015;35(3):1219-29. doi: 10.1159/000373945.

Species:

Mouse

Sample Type:

Conditioned Media (Mesenchymal Stem cells from Bone marrow, B6 mice)

View Publication

Degoul F, Borel M, Jacquemot N, et al. In vivo efficacy of melanoma internal radionuclide therapy with a (131) I-labelled melanin-targeting heteroarylcarboxamide molecule. Int J Cancer. 2013;133(5)1042-1053.

Species:

Mouse

Sample Type:

Tissue Lysate (melanoma tumor tissue)

View Publication

Degoul, Françoise, et al. "In vivo efficacy of melanoma internal radionuclide therapy with a 131I?labelled melanin?targeting heteroarylcarboxamide molecule." International journal of cancer 133.5 (2013): 1042-1053.

Species:

Mouse

Sample Type:

Tissue Lysate (B16 melanoma tumoural protein extracts)

View Publication

Brunner S., Huber B., Weinberger T., et al. Migration of bone marrow-derived cells and improved perfusion after treatment with erythropoietin in a murine model of myocardial infarction. J Cell Mol Med. 2012 Jan;16(1):152-9. doi: 10.1111/j.1582-4934.2011.01286.x.

Species:

Mouse

Sample Type:

Serum (EPO treated mice)

View Publication

Nasr M., Nafee N., Saad H., Kazem A. Improved antitumor activity and reduced cardiotoxicity of epirubicin using hepatocyte-targeted nanoparticles combined with tocotrienols against hepatocellular carcinoma in mice. European Journal of Pharmaceutics and Biopharmaceutics. Available online 6 May 2014, http://dx.doi.org.proxy.library.emory.edu/10.1016/j.ejpb.2014.04.016 [Epub ahead of print]

Species:

Mouse

Sample Type:

Tissue Lysate (Mouse Liver Lysates)

View Publication

Shields, Kristin M., et al. "Mitogen-activated protein kinase phosphatase-1 is a key regulator of hypoxia-induced vascular endothelial growth factor expression and vessel density in lung." The American journal of pathology 178.1 (2011): 98-109.

Species:

Mouse

Sample Type:

Cell Lysate

View Publication

Miguez-Pacheco, V., et al. "Development and characterization of niobium-releasing silicate bioactive glasses for tissue engineering applications." Journal of the European Ceramic Society 38.3 (2018): 871-876.

Species:

Mouse

Sample Type:

Conditioned Media (bone marros stromal stem cells)

View Publication

Bao, Zhengzheng, et al. "A Novel Cutaneous Fatty Acid-Binding Protein-Related Signaling Pathway Leading to Malignant Progression in Prostate Cancer Cells." Genes & cancer 4.7-8 (2013): 297-314.

Species:

Mouse

Sample Type:

Conditioned Media (LNCaP transfectants)

View Publication

George AL, Rajoria S, Suriano R, Mittleman A, Tiwari RK. Hypoxia and estrogen are functionally equivalent in breast cancer-endothelial cell interdependence. Molecular Cancer. 2012;11:80. doi:10.1186/1476-4598-11-80.

Species:

Mouse

Sample Type:

Conditioned Media (TG1-1 cells)

View Publication

Juffer P., Jaspers RT., Lips P., et al. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes. Am J Physiol Endocrinol Metab. 2012 Feb 15;302(4):E389-95. doi: 10.1152/ajpendo.00320.2011.

Species:

Mouse

Sample Type:

Conditioned Media (MLO-Y4 osteocytes cells)

View Publication

View All Publications For This Product

Reviews

Write a customer review

Great product

Rating
100%

The kit was packaged in an organized and clear manner, and it was easy to walk through each step of the procedure. I used the mouse IL-6, VEGF, and TNF-alpha kits for plasma as well as homogenized muscle samples. Each worked well, and I would be happy to use them again.

Review by Louis Hinkle,

from Houston Methodist Research Institute,

on Jan 26, 2020
Great product

Rating
80%

Great range of detection for mVEGF. Very straightforward and simple to use. Tear off strips make it convenient to conduct 2-3 assays.

Review by Martin Graf,

from VCU,

on Oct 5, 2018
Great product

Rating
80%

Wonderful product, used for analysis of cell culture supernates and serum samples from animal models. Very clean and easy to use.

Review by Nicole Malvin,

from Washington University,

on Jul 17, 2018

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