Quantibody® Mouse Cytokine Array 1 Kit. Detects 20 Mouse Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 20 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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$5 Amazon gift card in every kit box purchased.
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
|
IL-12 p70
| IL-13
| IL-17A
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-9
| KC (CXCL1)
|
MCP-1 (CCL2)
| M-CSF
| RANTES (CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
He S, Zhao J, Xu X, Cui X, Wang N, Han X, Guo Y, Liu Q. Uncovering the Molecular Mechanism of the Qiang-Xin 1 Formula on Sepsis-Induced Cardiac Dysfunction Based on Systems Pharmacology. Oxid Med Cell Longev. 2020 Aug 27;2020:3815185. doi: 10.1155/2020/3815185. PMID: 32908632; PMCID: PMC7474398.
Species:
Mouse
Sample Type:
Serum
Zingariello, Maria, et al. "Characterization of the TGF-?1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis." Blood 121.17 (2013): 3345-3363.
Species:
Mouse
Sample Type:
Bone Marrow Aspirate (Gata1-low mouse model of myelofibrosis)
Zingariello, Maria, et al. "Characterization of the TGF-?1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis." Blood 121.17 (2013): 3345-3363.
Species:
Mouse
Sample Type:
Conditioned Media (Gata1-low mouse model of myelofibrosis)
Arevalo-Martin A., Molina-Holgado E., Guaza C. A CB?/CB? receptor agonist, WIN 55,212-2, exerts its therapeutic effect in a viral autoimmune model of multiple sclerosis by restoring self-tolerance to myelin. Neuropharmacology. 2012 Sep;63(3):385-93. doi: 10.1016/j.neuropharm.2012.04.012.
Species:
Mouse
Sample Type:
Tissue Lysate (Mouse brain / CNS and spleen lysates)
Huggenberger R., et al. Stimulation of lymphangiogenesis via VEGFR-3 inhibits chronic skin inflammation. J Exp Med. 2010 Sep 27;207(10):2255-69. doi: 10.1084/jem.20100559.
Species:
Mouse
Sample Type:
Tissue Lysate (Tissue Lysate, Ear skin lysate)
Kelley P., et al. Regressed lymphatic vessels develop during corneal repair. Lab Invest. 2011 Nov;91(11):1643-51. doi: 10.1038/labinvest.2011.121.
Species:
Mouse
Sample Type:
Tissue Lysate (Corneal lysates)
Fink DM, Connor AL, Kelley PM, Steele MM, Hollingsworth MA, Tempero RM. Nerve Growth Factor Regulates Neurolymphatic Remodeling during Corneal Inflammation and Resolution. Ng LG, ed. PLoS ONE 2014;9(11):e112737. doi:10.1371/journal.pone.0112737.
Species:
Mouse
Sample Type:
Tissue Lysate (Corneal Lysates)
Huang X, Ren L, Ye P, Cheng C, Wu J, et al. (2014) Peroxisome Proliferator Activated Receptor c Deficiency in T Cells Accelerates Chronic Rejection by Influencing the Differentiation of CD4+ T Cells and Alternatively Activated Macrophages. PLoS ONE 9(11): e112953. doi:10.1371/journal.pone.0112953
Species:
Mouse
Sample Type:
Conditioned Media (Tissue infiltrating CD4 T cells stimed with anti=CD3)
Guo H., Yun C., Hou G., Du J., Huang X., et al. Mangiferin Attenuates Th1/Th2 Cytokine Imbalance in an Ovalbumin-Induced Asthmatic Mouse Model. PLOS One. Published: June 23, 2014. DOI: 10.1371/journal.pone.0100394
Species:
Mouse
Sample Type:
Serum
Vila` L, Roglans N, Baena M, Barroso E, Alegret M, et al. (2012) Metabolic Alterations and Increased Liver mTOR Expression Precede the Development of Autoimmune Disease in a Murine Model of Lupus Erythematosus. PLoS ONE 7(12): e51118. doi:10.1371/journal.pone.0051118
Species:
Mouse
Sample Type:
Conditioned Media (Splenocyte cultsures from control of SLE mice)
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Mouse Cytokine Array Q1I used the Mouse Cytokine Array Q1 to evaluate cell culture media samples from murine macrophages that were treated with various drug combinations. The array kit instructions were easy to follow and the overall process went very smoothly. The provided scanning service was also very helpful. I will definitely be recommending.
from The University of Memphis,
on
Mouse Liver Cytokine Array 1Utilized the quantibody mouse cytokine array 1 on liver samples from mice in a high-fat diet induced mouse model on 22 samples. It was easy, straight forward and they do the scanning for free.from University of Southern California,
on
Great productVery easy! I recommend their scanning service!!!from NIH,
on
Great productI used quite a a lot of cytokine and chemokine array kit from Raybiotech.These product is very simple and reliable to measure the production of cytokine/chemokine from cell culture media.They also provide a free Scanning service, so no need to worry about slide scanner. Highly recommended.from National Institute of health,
on
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