G-Series Mouse Cytokine Array 4000 Kit. A combination of 5 non-overlapping arrays to measure the relative expression levels of 200 mouse cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 16 Sample Kit, 32 Sample Kit, 64 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 200 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
4-1BB
(TNFRSF9/CD137)
|
6Ckine
(CCL21)
|
Ace
|
Activin A
|
ADAMTS-1
(METH1)
|
|
Adiponectin
(ACRP30)
|
ALK-1
|
Amphiregulin
|
ANGPTL1
|
ANGPTL3
|
|
Artemin
|
Axl
|
BAFF R
|
Betacellulin
(BTC)
|
bFGF
|
|
BLC
(CXCL13)
|
C5a
|
Cardiotrophin-1
(CT-1)
|
CCL28
(MEC)
|
CCL6
|
|
CD27
(TNFRSF7)
|
CD27 Ligand
(TNFSF7)
|
CD30
(TNFRSF8)
|
CD30 Ligand
(TNFSF8)
|
CD32a
|
|
CD36
(SR-B3)
|
CD40
(TNFRSF5)
|
CD40 Ligand
(TNFSF5)
|
CD48
(SLAMF2)
|
CD6
|
|
CD80
(B7-1)
|
Chemerin
|
Chordin
|
Clusterin
|
CRP
(C-Reactive Protein)
|
|
CTLA-4
(CD152)
|
CXCL16
|
Cystatin C
|
DAN
|
Decorin
|
|
DKK-1
|
DLL4
|
Dtk
|
E-Cadherin
|
EDAR
|
|
EGF
|
Endocan
|
Endoglin
(CD105)
|
Eotaxin-1
(CCL11)
|
Eotaxin-2
(MPIF-2/CCL24)
|
|
Epigen
|
Epiregulin
|
E-Selectin
|
Fas
(TNFRSF6/Apo-1)
|
Fas Ligand
(TNFSF6)
|
|
Fetuin A
|
Flt-3 Ligand
|
Fractalkine
(CX3CL1)
|
Galectin-1
|
Galectin-3
|
|
Galectin-7
|
Gas 1
|
Gas 6
|
GCSF
|
GITR
(TNFRSF18)
|
|
GITR Ligand
(TNFSF18)
|
GM-CSF
|
gp130
|
Granzyme B
|
Gremlin-1
|
|
H60
|
HAI-1
|
HGF
|
HGFR
|
I-309
(TCA-3/CCL1)
|
|
ICAM-1
(CD54)
|
IFN-gamma
|
IFN-gamma R1
|
IGF-1
|
IGFBP-2
|
|
IGFBP-3
|
IGFBP-5
|
IGFBP-6
|
IL-1 alpha
(IL-1 F1)
|
IL-1 beta
(IL-1 F2)
|
|
IL-1 R4
(ST2)
|
IL-1 ra
(IL-1 F3)
|
IL-10
|
IL-12 p40
|
IL-12 p70
|
|
IL-13
|
IL-15
|
IL-17 RB
|
IL-17A
|
IL-17B
|
|
IL-17E
(IL-25)
|
IL-17F
|
IL-2
|
IL-2 R alpha
|
IL-20
|
|
IL-21
|
IL-22
|
IL-23 p19
|
IL-28A
(IFN-lambda 2)
|
IL-3
|
|
IL-3 R beta
|
IL-33
(IL-1 F11)
|
IL-4
|
IL-5
|
IL-6
|
|
IL-7
|
IL-7 R alpha
|
IL-9
|
I-TAC
(CXCL11)
|
JAM-A
(CD321/F11R)
|
|
KC
(CXCL1)
|
Kremen-1
|
Leptin
|
Leptin R
|
Limitin
|
|
Lipocalin-2
(NGAL)
|
LIX
|
LOX-1
|
L-Selectin
(CD62L)
|
Lungkine
(CXCL15)
|
|
Lymphotactin
(XCL1)
|
MAdCAM-1
|
Marapsin
|
MBL-2
|
MCP-1
(CCL2)
|
|
MCP-5
|
M-CSF
|
MDC
(CCL22)
|
Meteorin
|
MFG-E8
|
|
MIG
(CXCL9)
|
MIP-1 alpha
(CCL3)
|
MIP-1 beta
(CCL4)
|
MIP-1 gamma
|
MIP-2
|
|
MIP-3 alpha
(CCL20)
|
MIP-3 beta
(CCL19)
|
MMP-10
|
MMP-2
|
MMP-3
|
|
Neprilysin
|
Nope
|
NOV
(CCN3)
|
Osteoactivin
(GPNMB)
|
Osteopontin
(SPP1)
|
|
Osteoprotegerin
(TNFRSF11B)
|
OX40 Ligand
|
P-Cadherin
|
PDGF-AA
|
Pentraxin-3
(TSG-14)
|
|
Periostin
|
Persephin
|
Platelet Factor 4
(CXCL4)
|
PlGF-2
|
Progranulin
|
|
Prolactin
|
Pro-MMP-9
|
Prostasin
|
P-Selectin
|
RAGE
|
|
RANTES
(CCL5)
|
Renin 1
|
Resistin
|
SCF
|
SDF-1 alpha
(CXCL12 alpha)
|
|
sFRP-3
|
SLAM
(CD150)
|
Sonic Hedgehog N-Terminal
(Shh-N)
|
TACI
(TNFRSF13B)
|
TARC
(CCL17)
|
|
TCK-1
(CXCL7)
|
TECK
(CCL25)
|
Testican 3
|
TGF beta 1
|
Thrombopoietin
(TPO)
|
|
TIM-1
(KIM-1)
|
TNF alpha
|
TNF RI
(TNFRSF1A)
|
TNF RII
(TNFRSF1B)
|
TRAIL
(TNFSF10)
|
|
Trance
(TNFSF11)
|
TREM-1
|
TREML1
(TLT-1)
|
TROY
(TNFRSF19)
|
Tryptase epsilon
|
|
TSLP
|
TWEAK
(TNFSF12)
|
TWEAK R
(TNFRSF12)
|
VCAM-1
(CD106)
|
VEGF-A
|
|
VEGF-B
|
VEGF-D
|
VEGFR1
|
VEGFR2
|
VEGFR3
|
Species Detected
Mouse
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Detection of Relative Protein Expression
- Detecting Patterns of Cytokine Expression
- Biomarker Screening
- Identifying Key Factors
- Confirming a Biological Process
Kit Components
- Antibody Array Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Lou F., Sun Y., Xu Z., et al. Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. Immunity . 2020 Jul 14;53(1):204-216.e10. doi: 10.1016/j.immuni.2020.06.004. Epub 2020 Jun 17.
Species:
Mouse
Sample Type:
Tissue Lysate (Skin samples in psoriasis model)
Wang X., Sun G., Feng T., et al. Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression. Cell Research volume 29, pages 787-803 (2019)
Species:
Mouse
Sample Type:
Tissue Lysate (brain tissue lysates from AD and normal patients)
Minmin Jiang, Wenying Cai, Junmin Zhang, Ting Xie, Liyan Xi, Xiqing Li, Jiufeng Sun, Melanization of a meristematic mutant of Fonsecaea monophora increase the pathogenesis in a BALB/c mice infection model, Medical Mycology, Volume 56, Issue 8, November 2018, Pages 979-986, https://doi.org/10.1093/mmy/myx148
Species:
Mouse
Sample Type:
Serum
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