RayBio® G-Series Mouse Cytokine Antibody Array 1000. A combination of Mouse Cytokine Antibody Array G3 & G4. Detects 96 Mouse Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 96 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Shipping Type | Blue ice |
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Scroll over each target protein for more information |
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Axl
|
bFGF
|
BLC
(CXCL13)
|
CD30 Ligand
(TNFSF8)
|
CD30
(TNFRSF8)
|
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CD40
(TNFRSF5)
|
CRG-2
|
CTACK
(CCL27)
|
CXCL16
|
CD26
(DPPIV)
|
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Dtk
|
Eotaxin-1
(CCL11)
|
Eotaxin-2
(MPIF-2/CCL24)
|
E-Selectin
|
Fas Ligand
(TNFSF6)
|
|
Fc gamma RIIB
(CD32b)
|
Flt-3 Ligand
|
Fractalkine
(CX3CL1)
|
GCSF
|
GITR
(TNFRSF18)
|
|
GM-CSF
|
HGFR
|
ICAM-1
(CD54)
|
IFN-gamma
|
IGFBP-2
|
|
IGFBP-3
|
IGFBP-5
|
IGFBP-6
|
IGF-1
|
IGF-2
|
|
IL-1 beta
(IL-1 F2)
|
IL-10
|
IL-12 p40/p70
|
IL-12 p70
|
IL-13
|
|
IL-15
|
IL-17A
|
IL-17 RB
|
IL-1 alpha
(IL-1 F1)
|
IL-2
|
|
IL-3
|
IL-3 R beta
|
IL-4
|
IL-5
|
IL-6
|
|
IL-7
|
IL-9
|
I-TAC
(CXCL11)
|
KC
(CXCL1)
|
Leptin
|
|
Leptin R
|
LIX
|
L-Selectin
(CD62L)
|
Lungkine
(CXCL15)
|
Lymphotactin
(XCL1)
|
|
MCP-1
(CCL2)
|
MCP-5
|
M-CSF
|
MDC
(CCL22)
|
MIG
(CXCL9)
|
|
MIP-1 alpha
(CCL3)
|
MIP-1 gamma
|
MIP-2
|
MIP-3 beta
(CCL19)
|
MIP-3 alpha
(CCL20)
|
|
MMP-2
|
MMP-3
|
Osteopontin
(SPP1)
|
Osteoprotegerin
(TNFRSF11B)
|
Platelet Factor 4
(CXCL4)
|
|
Pro-MMP-9
|
P-Selectin
|
RANTES
(CCL5)
|
Resistin
|
SCF
|
|
SDF-1 alpha
(CXCL12 alpha)
|
Sonic Hedgehog N-Terminal
(Shh-N)
|
TNF RI
(TNFRSF1A)
|
TNF RII
(TNFRSF1B)
|
TARC
(CCL17)
|
|
I-309
(TCA-3/CCL1)
|
TECK
(CCL25)
|
TCK-1
(CXCL7)
|
TIMP-1
|
TIMP-2
|
|
TNF alpha
|
Thrombopoietin
(TPO)
|
TRANCE
(TNFSF11)
|
TROY
(TNFRSF19)
|
TSLP
|
|
VCAM-1
(CD106)
|
VEGF-A
|
VEGFR1
|
VEGFR2
|
VEGFR3
|
|
VEGF-D
|
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Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array G1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Koszalka P., Golunska M., Stanislawowski M., et al. CD73 on B16F10 melanoma cells in CD73-deficient mice promotes tumor growth, angiogenesis, neovascularization, macrophage infiltration and metastasis. Int J Biochem Cell Biol. 2015 Dec;69:1-10. doi: 10.1016/j.biocel.2015.10.003.
Species:
Mouse
Sample Type:
Tissue Lysate (B16F10 melanoma model)
Ionescu L, Byrne RN, van Haaften T, et al. Stem cell conditioned medium improves acute lung injury in mice: in vivo evidence for stem cell paracrine action. American Journal of Physiology - Lung Cellular and Molecular Physiology. 2012;303(11):L967-L977. doi:10.1152/ajplung.00144.2011.
Species:
Mouse
Sample Type:
Conditioned Media (Mesenchymal Stem Cell and Fibroblast media)
Litvinov D., et al. Anti-atherosclerotic actions of azelaic acid, an end product of linoleic acid peroxidation, in mice. Atherosclerosis. 2010 Apr;209(2):449-54. doi: 10.1016/j.atherosclerosis.2009.09.076.
Species:
Mouse
Wittle U., Schmidt A., Poxleitner P., et al. The chemokine ligand CXCL16 is an indicator of bacterial infection in necrotizing pancreatitis. Pancreatology. 2015 Mar-Apr;15(2):124-30. doi: 10.1016/j.pan.2015.01.004.
Species:
Mouse
Sample Type:
Serum (Pooled sera samples from bacterially infected mice)
Miyauchi S., et al. Oral infection with Porphyromonas gingivalis and systemic cytokine profile in C57BL/6.KOR-ApoEshl mice. J Periodontal Res. 2012 Jun;47(3):402-8. doi: 10.1111/j.1600-0765.2011.01441.x
Species:
Mouse
Miyauchi S., Maekawa T., Aoki Y., et al. Oral infection with Porphyromonas gingivalis and systemic cytokine profile in C57BL/6.KOR-ApoE shl mice. J Periodontal Res. 2012 Jun;47(3):402-8. doi: 10.1111/j.1600-0765.2011.01441.x.
Species:
Mouse
Sample Type:
Serum (oral infectioni with Porphyromonas gingivalis and systemic cytokine profiling)
Chen, D., Wang, R., Long, M., Li, W., Xiao, B., Deng, H. ... Hao, W. (2021). Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11. Oncology Reports, 45, 535-546.
Species:
Mouse
Sample Type:
Serum (cell culture media)
Labelle M., et al. Platelets guide the formation of early metastatic niches. PNAS published online July 2014. www.pnas.org/cgi/doi/10.1073/pnas.1411082111
Species:
Mouse
Sample Type:
Conditioned Media (platelet releasates)
Narasimhulu CA., Selvarajan K., Litvinov D., Parthasarathy S. Anti-Atherosclerotic and Anti-Inflammatory Actions of Sesame Oil. J Med Food 18 (1) 2015, 11-20 DOI: 10.1089/jmf.2014.0138
Species:
Mouse
Sample Type:
Plasma (Seasame oil treated mice)
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