RayBio® C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 42 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
ENA-78 (CXCL5)
| GCSF
| GM-CSF
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
|
I-309 (TCA-3/CCL1)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-7
| IL-8 (CXCL8)
|
IL-10
| IL-12 p40/p70
| IL-13
| IL-15
| IFN-gamma
|
MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| M-CSF
| MDC (CCL22)
|
MIG (CXCL9)
| MIP-1 delta (CCL15)
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TNF alpha
| TNF beta (TNFSF1B)
| EGF
|
IGF-1
| Angiogenin
| Oncostatin M
| Thrombopoietin (TPO)
| VEGF-A
|
PDGF-BB
| Leptin
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Marks DJB, Harbord MWN, MacAllister R, Rahman FZ, Young J, et al. Defective acute inflammation in Crohn's disease: a clinical investigation. Lancet. 2006;367:668-678.
Species:
Human
Sample Type:
Tissue Lysate (Supernates of forearm epidermal "skin windows")
Formentini, Laura, et al. "Mitochondrial H+-ATP synthase in human skeletal muscle: contribution to dyslipidaemia and insulin resistance." Diabetologia 60.10 (2017): 2052-2065.
Species:
Human
Sample Type:
Tissue Lysate (skeletal muscle)
Wanichkul T., Han S., Huang RP., Sidell N. Cytokine regulation by peroxisome proliferator-activated receptor gamma in human endometrial cells. Fertil Steril. 2003 Mar;79 Suppl 1:763-9.
Species:
Human
Sample Type:
Conditioned Media (Endometrial Cell conditioned media)
Braun, Jennifer, et al. "Endothelial cell ephrinB2-dependent activation of monocytes in arteriosclerosis." Arteriosclerosis, thrombosis, and vascular biology 31.2 (2011): 297-305.
Species:
Human
Sample Type:
Conditioned Media
Furukawa, Kanako, et al. "Increase of nitrosative stress in patients with eosinophilic pneumonia." Respiratory research 12.1 (2011): 81.
Species:
Human
Sample Type:
Exhaled Breath Condensate
Chae, M., Son, E.D., Bae, IH. et al. UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes. Exp Mol Med 52, 293-307 (2020). https://doi.org/10.1038/s12276-020-0388-y
Species:
Human
Sample Type:
Conditioned Media (supernatants)
Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007;43:1117-1124.
Species:
Human
Sample Type:
Conditioned Media (Breast cancer cell-cultured medium)
Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007;43:1117-1124.
Species:
Human
Sample Type:
Serum (Breast cancer patient serum)
Gaceb, Abderahim, et al. "Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB." Journal of Cerebral Blood Flow & Metabolism 38.1 (2018): 45-57.
Species:
Human
Sample Type:
Conditioned Media (human pericytes)
DeCicco KL, Tanaka T, Andreola F, De Luca LM. The effect of thalidomide on non-small cell lung cancer (NSCLC) cell lines: possible involvement of the PPAR-gamma pathway. Carcinogenesis. 2004;25:1805-1812.
Species:
Human
Sample Type:
Conditioned Media (Non-small cell lung cancer cell lines, NCI-H460 or NCI-H1299)
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Great productThe array is very easy to follow. It comes with very through instruction and pictures. I have been away from experiments for several years. Being back to lab after those years, this very detailed and highly organized assay made my life so much easier. Wish every test package comes like that
from University of the Pacific,
on
Great productEasy to use and reliablefrom Dana Farber Cancer Institute,
on
Great productThis was exceptionally easy to run and imageon
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