RayBio® Human SDF-1 alpha (CXCL12 alpha) ELISA Kit for cell culture supernatants and heparin, EDTA, or citrate-treated plasma samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Human |
| Accession Number | P48061 |
| Gene Id | 6387 |
| Gene Symbols | CXCL12|SDF1|SDF1A|SDF1B |
| Protein Name / Synonyms | Stromal cell-derived factor 1 (SDF-1) (hSDF-1) (C-X-C motif chemokine 12) (Intercrine reduced in hepatomas) (IRH) (hIRH) (Pre-B cell growth-stimulating factor) (PBSF) [Cleaved into: SDF-1-beta(3-72) SDF-1-alpha(3-67)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 42 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 93.75 pg/ml - 6000 pg/ml |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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SDF-1 in cell cutureIt is excellent kit. Very easy to follow. I got a perfect standard curve all the times i worked with it. Highly recommended for SDF-1 alpha analysis.
on
Great productThe product is best to know the secretion of SDF-1 factor from cancer cells. The manual is easy to understand and follow. I am using this Kit to know the secretion of SDF-1 from interleukin-stimulated adenocarcinoma cells.from Temple University School of Pharamacy,
on
Great productExcellent kit with all of the needed reagents. Takes about 5 hours, plus however long it takes you for washes and reagent addition, so figure about 6.5 hours start to finish if you are doing everything manually.I like the fact the wells are in 8 well strips, so you can tailor the number of assays you have to do in a given run. However, the rack the strips snap into weakens over time, so the strips will start falling out after about 3 uses, which can make it hard to dump the washes.Very specific to SDF1a (CXCL12a). I tired all 5 variants and there is minimal cross reactivity to the others.Looses a star because I couldn't ever get the standard curve to work correctly. Consistently the four highest concentration samples would max out the signal, but all the other concentrations were very linear.from Modern Vector,
on
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