RayBio® C-Series Mouse Cytokine Antibody Array 1 Kit. Detects 22 Mouse Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 22 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
GCSF
|
GM-CSF
|
IL-2
|
IL-3
|
IL-4
|
|
IL-5
|
IL-6
|
IL-9
|
IL-10
|
IL-12 p40/p70
|
|
IL-12 p70
|
IL-13
|
IL-17A
|
IFN-gamma
|
MCP-1
(CCL2)
|
|
MCP-5
|
RANTES
(CCL5)
|
SCF
|
TNF RI
(TNFRSF1A)
|
TNF alpha
|
|
Thrombopoietin
(TPO)
|
VEGF-A
|
|||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Wilson, Andrew A., et al. "Lentiviral delivery of RNAi for in vivo lineage-specific modulation of gene expression in mouse lung macrophages." Molecular Therapy 21.4 (2013): 825-833.
Species:
Mouse
Sample Type:
Conditioned Media (Raw 264.7 cells)
Chae M., Peterson TE., Balgeman A., et al. Increasing glioma-associated monocytes leads to increased intratumoral and systemic myeloid-derived suppressor cells in a murine model. Neuro Oncol. 2014 Dec 23. pii: nou343. [Epub ahead of print]
Species:
Mouse
Sample Type:
Conditioned Media (Glioma, CL261 cells)
Denis Gallagher, Andreea A. Norman, Cameron L. Woodard, et al. "Transient Maternal IL-6 Mediates Long-Lasting Changes in Neural Stem Cell Pools by Deregulating an Endogenous Self-Renewal Pathway." Cell Stem Cell 13, 564-576, November 7, 2013. http://dx.doi.org/10.1016/j.stem.2013.10.002
Species:
Mouse
Sample Type:
Conditioned Media (E12.5 and E13.5 embryonic cells)
Trial, JoAnn, et al. "Th1/M1 conversion to th2/m2 responses in models of inflammation lacking cell death stimulates maturation of monocyte precursors to fibroblasts." Frontiers in immunology 4 (2013): 287.
Species:
Mouse
Sample Type:
Tissue Lysate (heart)
Martinet W., Verheye S., De Meyer I., et al. Everolimus triggers cytokine release by macrophages: rationale for stents eluting everolimus and a glucocorticoid. Arterioscler Thromb Vasc Biol. 2012 May;32(5):1228-35. doi: 10.1161/ATVBAHA.112.245381.
Species:
Mouse
Sample Type:
Conditioned Media (Primary differentiated macophages with everolimus treatment)
De Meyer I., Martinet W., Schrijvers DM., et al. Toll-like receptor 7 stimulation by imiquimod induces macrophage autophagy and inflammation in atherosclerotic plaques. Basic Res Cardiol. 2012 May;107(3):269. doi: 10.1007/s00395-012-0269-1.
Species:
Mouse
Sample Type:
Conditioned Media (BM-macs treated with TLR7)
Kobayashi T., Tanaka K., Fujita T., et al. Bidirectional role of IL-6 signal in pathogenesis of lung fibrosis. Respir Res. 2015 Aug 20;16:99. doi: 10.1186/s12931-015-0261-z
Species:
Mouse
Sample Type:
Conditioned Media (Primary alveolar epithelial cells stimulated with bleomycin)
Cieslik, Katarzyna A., et al. "Immune-inflammatory dysregulation modulates the incidence of progressive fibrosis and diastolic stiffness in the aging heart." Journal of molecular and cellular cardiology 50.1 (2011): 248-256.
Species:
Mouse
Sample Type:
Tissue lysate (whole heart)
Resa-Infante P., Thieme R., Ernst T., Arck P., Ittrich H., Reimer R., Gabriel G. Importin-?7 is Required for Enhanced Influenza A Virus Replication in the Alveolar Epithelium and Severe Lung Damage in Mice. Journal of Virology Volume 88, Issue 11.
Species:
Mouse
Sample Type:
Tissue Lysate (Lung Tissue Lysate)
Loria, Gilbert David, et al. "Platelets support a protective immune response to LCMV by preventing splenic necrosis." Blood 121.6 (2013): 940-950.
Species:
Mouse
Sample Type:
Serum (platelet-depleted mice infected with the mouse arenavirus lymphocytic choriomeningitis virus)
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Great productExceptionally easy and reproducible. Highly recommended.
from CWRU,
on
Great productThank you for the product. It worked really well with our samples. I used overnight incubation and I am happy with the result. One of our labmates have ordered another Cytokine array after seeing it working for me.from Albert Einstein College of Medicine,
on
Great productclear spots, convenient to use, very good product with high quality and relatively low price.on
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