RayBio® C-Series Mouse Angiogenesis Antibody Array 1 Kit. Detects 24 Mouse Angiogenic Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 24 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | Angiogenesis |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| Fas Ligand (TNFSF6)
| bFGF
| GCSF
| GM-CSF
|
IFN-gamma
| IGF-2
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-12 p40/p70
|
IL-12 p70
| IL-13
| IL-6
| IL-9
| Leptin
|
MCP-1 (CCL2)
| M-CSF
| MIG (CXCL9)
| Platelet Factor 4 (CXCL4)
| TIMP-1
|
TIMP-2
| TNF alpha
| Thrombopoietin (TPO)
| VEGF-A
| |
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Angiogenesis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Su VY, Chiou SH, Lin CS, Mo MH, Yang KY. Induced Pluripotent Stem Cells Attenuate Endothelial Leakage in Acute Lung Injury via Tissue Inhibitor of Metalloproteinases-1 to Reduce Focal Adhesion Kinase Activity. Stem Cells. 2019 Dec;37(12):1516-1527. doi: 10.1002/stem.3093. Epub 2019 Nov 18. PMID: 31588644.
Species:
Mouse
Sample Type:
Conditioned media (cell culture media, murin-induced pluripotent stem cells (miPSCs))
Cheng YC, Chiang HY, Cheng SJ, Chang HW, Li YJ, Shieh SY. Loss of the tumor suppressor BTG3 drives a pro-angiogenic tumor microenvironment through HIF-1 activation. Cell Death Dis. 2020 Dec 11;11(12):1046. doi: 10.1038/s41419-020-03248-5. PMID: 33311481; PMCID: PMC7732837.
Species:
Mouse
Sample Type:
Serum
Schaafhausen, Maximilian K., et al. "Tumor angiogenesis is caused by single melanoma cells in a manner dependent on reactive oxygen species and NF-?B." J Cell Sci 126.17 (2013): 3862-3872.
Species:
Mouse
Sample Type:
Conditioned Media (mouse melanocytes)
Adini I., Adini A., Bazinet L., et al. Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential. FASEB J. 2014 Nov 18. pii: fj.14-255398.
Species:
Mouse
Sample Type:
Conditioned Media (pigemented and nonpigmented melanocytes)
Caso G., et al. Dysregulation of CXCL9 and reduced tumor growth in Egr-1 deficient mice. J Hematol Oncol. 2009 Feb 7;2:7. doi: 10.1186/1756-8722-2-7.
Species:
Mouse
Sample Type:
Tissue Lysate (Tumor tissue)
Huang H., Haq O., Utsumi T., et al. Intestinal and plasma VEGF levels in cirrhosis: the role of portal pressure. J Cell Mol Med. 2012 May;16(5):1125-33. doi: 10.1111/j.1582-4934.2011.01399.x.
Species:
Rat
Sample Type:
Plasma (Cirrhotic and non-ascititc rat model)
Zhang J., et al. Chemopreventive Effect of Korean Angelica Root Extract on TRAMP Carcinogenesis and Integrative “Omic†Profiling of Affected Neuroendocrine Carcinomas. Molecular Carcinogenesis October 2014, epub ahead of print. DOI 10.1002/mc.22230
Species:
Mouse
Sample Type:
Cell Lysate (Neruoendocrine Carcinoma)
Giddabasappa A., Eswaraka J., Barrett C., et al. ?-LGND2, an ER? selective agonist, inhibits pathologic retinal neovascularization. Invest Ophthalmol Vis Sci. 2012 Jul 31;53(8):5066-75. doi: 10.1167/iovs.12-9627.
Species:
Mouse
Sample Type:
Tissue Lysate (Retinal Lysates samples)
Licarete E, Rauca VF, Luput L, Patras L, Sesarman A, Banciu M. The prednisolone phosphate?induced suppression of the angiogenic function of tumor?associated macrophages enhances the antitumor effects of doxorubicin on B16.F10 murine melanoma cells in vitro. Oncol Rep. 2019 Dec;42(6):2694-2705. doi: 10.3892/or.2019.7346. Epub 2019 Oct 1. PMID: 31578578.
Species:
Human
Sample Type:
Cell Lysate (Cell co-culture)
Mabeta P., Pepper M. Altered expression of platelet factor 4 and basic fibroblast growth factor correlates with the inhibition of tumor growth in mice. Biomedicine & Pharmacotherapy 69 (2015) 186-190
Species:
Mouse
Sample Type:
Plasma (Taxol and control treated mice)
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