Quantibody® Human Obesity Array 3 Kit. Detects 40 Human Adipokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 40 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Cardiovascular Disease, Obesity, Akt Signaling, AMPK Signaling, MAPK Signaling, mTOR Signaling, PI3K-AKT Signaling, p53 Signaling, NFkB Signaling |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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$5 Amazon gift card in every kit box purchased.
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Adiponectin (ACRP30)
| Adipsin (Complement Factor D)
| AgRP
| ANGPTL4
| BDNF
|
Chemerin
| CRP (C-Reactive Protein)
| Growth Hormone
| IFN-gamma
| IGFBP-1
|
IGFBP-2
| IGF-1
| IL-10
| IL-12 p40
| IL-12 p70
|
IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
| IL-6
| IL-8 (CXCL8)
| Insulin
|
Leptin
| Lipocalin-2 (NGAL)
| MSP alpha/beta
| Osteoprotegerin (TNFRSF11B)
| PAI-1
|
PDGF-BB
| Pepsinogen 1
| Pepsinogen 2
| Procalcitonin
| Prolactin
|
RANTES (CCL5)
| RBP4
| Resistin
| SAA (Serum Amyloid A)
| TGF beta 1
|
Thrombospondin 1
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Obesity Array Q3 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Dieli-Conwright, C.M., Parmentier, JH., Sami, N. et al. Adipose tissue inflammation in breast cancer survivors: effects of a 16-week combined aerobic and resistance exercise training intervention. Breast Cancer Res Treat 168, 147-157 (2018). https://doi.org/10.1007/s10549-017-4576-y
Species:
Human
Sample Type:
Conditioned media (Adipose tissue)
Lin, Yung-Kuo, et al. "Heart failure epicardial fat increases atrial arrhythmogenesis." International journal of cardiology 167.5 (2013): 1979-1983.
Species:
Rabbit
Sample Type:
Tissue Lysate (Epicardial fat tissues obtained from HF rabbits and control rabbits)
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