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    Human Thrombospondin 1 ELISA Kit

    Rating:
    80 % of 100
    4  Reviews

    RayBio® Human Thrombospondin 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

    Documents
    As low as $420.00
    In stock
    Only %1 left
    Catalog #:
    ELH-TSP1
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human
    Accession Number P07996
    Gene Id 7057
    Gene Symbols THBS1|TSP|TSP1
    Protein Name / Synonyms Thrombospondin-1
    Quantitative/Semi-Quantitative Quantitative
    Specificity This ELISA antibody pair detects human Thrombospondin-1. It shows less than 5% cross-reactivity with recombinant human Thrombospondin-2 and recombinant human Thrombospondin-4.
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 6 ng/ml

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    Detection Range 6 ng/ml - 2000 ng/ml
    Recommended Dilution (Serum/Plasma) 10 - 200 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage ≤-20°C

    Risk-Free Guarantee

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    $5 Amazon gift card in every kit box purchased.

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Reviews

    Write a customer review
    1. Scientist
      Rating
      100%
      Sample: media collected from cells of central nervous system
      This kit works very nice. I have reproducible data using the kit. The background is almost nothing. The standard curves were very nice too. Strongly recommend!

      Review by

      from Massachusette General Hospital,

      on

    2. Great product
      Rating
      80%
      Protocol was straightforward. I would recommend to the company to use a better naming system for the reagents (each item having 2 names, both letters, was a bit confusing).

      Review by

      from Houston Methodist,

      on

    3. Great product
      Rating
      60%
      Standards worked fine but couldn't detect molecule in sample.

      Review by

      from University of Houston,

      on

    4. Great product
      Rating
      80%
      I used this kit to test the protein levels in conditioned media. I used 10% media diluted in the included buffer. The protocol is easy to follow and results were accurate with what we've seen in other assays. The only problem that I had was that it's difficult to do the washes because of the 8-well strips. I used the whole plate at once, but was unable to shake the liquid out of the plate because the strips would fall off when I did this.

      Review by

      from Indiana University School of Medicine,

      on

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