Membrane-based inflammation cytokine array detects 40 human inflammatory factors.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | Inflammation |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Eotaxin-1
(CCL11)
|
Eotaxin-2
(MPIF-2/CCL24)
|
GCSF
|
GM-CSF
|
ICAM-1
(CD54)
|
|
IFN-gamma
|
I-309
(TCA-3/CCL1)
|
IL-1 alpha
(IL-1 F1)
|
IL-1 beta
(IL-1 F2)
|
IL-2
|
|
IL-3
|
IL-4
|
IL-6
|
IL-6 R
|
IL-7
|
|
IL-8
(CXCL8)
|
IL-10
|
IL-11
|
IL-12 p40
|
IL-12 p70
|
|
IL-13
|
IL-15
|
IL-16
|
IL-17A
|
IP-10
(CXCL10)
|
|
MCP-1
(CCL2)
|
MCP-2
(CCL8)
|
M-CSF
|
MIG
(CXCL9)
|
MIP-1 alpha
(CCL3)
|
|
MIP-1 beta
(CCL4)
|
MIP-1 delta
(CCL15)
|
RANTES
(CCL5)
|
TGF beta 1
|
TNF alpha
|
|
TNF beta
(TNFSF1B)
|
TNF RI
(TNFRSF1A)
|
TNF RII
(TNFRSF1B)
|
PDGF-BB
|
TIMP-2
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Inflammation Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Rooney P., Srivastava A., Watson L., et al. Hyaluronic acid decreases IL-6 and IL-8 secretion and permeability in an inflammatory model of interstitial cystitis. Acta Biomater. 2015 Jun;19:66-75. doi: 10.1016/j.actbio.2015.02.030.
Species:
Human
Sample Type:
Cell Lysate (HTB4 cells)
Chan J., Tan M., Sng M., et al. Cancer-associated fibroblasts enact field cancerization by promoting extratumoral oxidative stress. Cell Death Dis 8 (1), e2562. 2017 Jan 19.
Species:
Human
Sample Type:
Conditioned Media (Cancer associated fibroblast cells in squamous cell carcinoma)
Xing W., Xiao Y., Lu., et al. GFI1 downregulation promotes inflammation-linked metastasis of colorectal cancer. Cell Death Differ. 2017 May; 24(5): 929-943.
Species:
Human
Sample Type:
Conditioned Media (Various cancer cell lines)
Kohler S., Bethke N., Bothe M., et al. The early cellular signatures of protective immunity induced by live viral vaccination. Eur J Immunol. 2012 Sep;42(9):2363-73. doi: 10.1002/eji.201142306
Species:
Human
Sample Type:
Serum (Live viral vaccine model of cellular immunity)
Baldwin, John, et al. "Susceptibility of human iris stromal cells to herpes simplex virus 1 entry." Journal of virology 87.7 (2013): 4091-4096.
Species:
Human
Sample Type:
Conditioned Media (HSV-1 infection of HIS cells inflammatory response)
Fierro NA, Pedraza-Alva G, Rosenstein Y. TCR-Dependent Cell Response is Modulated by the Timing of CD43 Engagement. The Journal of Immunology June 15, 2006 vol. 176 no. 12 7346-7353
Species:
Human
Sample Type:
Conditioned Media (Conditioned Media)
Ebihara N, Yamagami S, Yokoo S, Amano S, Murakami A. Involvement of C-C Chemokine Ligand 2-CCR2 Interaction in Monocyte-Lineage Cell Recruitment of Normal Human Corneal Stroma. J Immunol. 2007;178:3288-3292.
Species:
Human
Sample Type:
Conditioned Media (Keratocyte cell-cultured medium)
McGovern NN, Cowburn AS, Porter L, Walmsley SR, Thompson AAR, et al. Hypoxia Selectively Inhibits Respiratory Burst Activity and Killing of Staphylococcus aureus in Human Neutrophils. J Immunol. 2011;186:453-463.
Species:
Human
Sample Type:
Conditioned Media (Neutrophils with/wo hypoxia)
Fernandez-Pisonero I., Duenas Al., Barreiro O., et al. Lipopolysaccharide and sphingosine-1-phosphate cooperate to induce inflammatory molecules and leukocyte adhesion in endothelial cells. J Immunol. 2012 Dec 1;189(11):5402-10. doi: 10.4049/jimmunol.1201309
Species:
Human
Sample Type:
Conditioned Media (Human umbilical vein endothelial (HUVEC) cell-cultured medium)
Fougeray S., Mami I., Bertho G., et al. Tryptophan depletion and the kinase GCN2 mediate IFN-?-induced autophagy. J Immunol. 2012 Sep 15;189(6):2954-64. doi: 10.4049/jimmunol.1201214.
Species:
Human
Sample Type:
Conditioned Media (Human Renal epithelial cells)
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