Quantibody® Human Chemokine Array 1 Kit. Detects 40 Human Chemokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 40 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
6Ckine (CCL21)
| Axl
| Betacellulin (BTC)
| CCL28 (MEC)
| CTACK (CCL27)
|
CXCL16
| ENA-78 (CXCL5)
| Eotaxin-3 (CCL26)
| GCP-2 (CXCL6)
| GRO
|
HCC-1 (CCL14)
| HCC-4 (CCL16)
| IL-9
| IL-17F
| IL-18 BP alpha
|
IL-28A
| IL-29
| IL-31
| IP-10 (CXCL10)
| I-TAC (CXCL11)
|
LIF
| Light (TNFSF14)
| Lymphotactin (XCL1)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| MDC (CCL22)
| MIF
| MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
|
MPIF-1 (CCL23)
| MSP alpha/beta
| NAP-2 (PPBP/CXCL7)
| Osteopontin (SPP1)
| PARC (CCL18)
|
Platelet Factor 4 (CXCL4)
| SDF-1 alpha (CXCL12 alpha)
| TARC (CCL17)
| TECK (CCL25)
| TSLP
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Chemokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.
Species:
Human
Sample Type:
Vitreous Fluid (Vitreous sample from retinopathy patients)
Velez G., Roybal C., Colgan D., et al. Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease. JAMA Ophthalmol. 2016 Apr;134(4):444-8. doi: 10.1001/jamaophthalmol.2015.5934.
Species:
Human
Sample Type:
Vitreous Fluid (Human Vitreous Fluid samples)
Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.
Species:
Human
Sample Type:
Conditioned Media (Embryonic stem cell derived astrocytes in immunodeficient mice)
Yang B., Deng Q., Zhang W., et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep. 2016 Jan 13;6:19272. doi: 10.1038/srep19272.
Species:
Human
Sample Type:
Plasma (Polycyclic aromatic hydrocarbon exposed patients)
Reategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094
Species:
Human
Sample Type:
Conditioned Media (Neutrophil swarming experiments)
Leung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327
Species:
Human
Sample Type:
Conditioned Media (A375 ERK2 treated cells with or without ddoxycycline)
Chen YL., Huang KF., Kuo WC., et al. Inhibition of glutaminyl cyclase attenuates cell migration modulated by monocyte chemoattractant proteins. Biochem. J. (2012) 442, 403-412. doi:10.1042/BJ20110535
Species:
Human
Sample Type:
Conditioned Media (U937 Cells)
Chowdhury UR., et al. Proteome Analysis of Human Aqueous Humor. Invest Ophthalmol Vis Sci. 2010 Oct;51(10):4921-31. doi: 10.1167/iovs.10-5531.
Species:
Human
Sample Type:
(other body fluid)
Laudanski P., Lemancewicz A., Kuc P., Charkiewicz K., et al. Chemokines Profiling of Patients with Preterm Birth. Mediators of Inflammation Volume 2014, Article ID 185758, 7 pages
Species:
Human
Sample Type:
Serum (Human Serum)
Laudanski P., Zbucka-Kretowska M., Charkiewicz K., et al. Maternal Plasma and Amniotic Fluid Chemokines Screening in Fetal Down Syndrome. Mediators of Inflammation Volume 2014, Article ID 835837, 10 pages. http://dx.doi.org/10.1155/2014/835837
Species:
Human
Sample Type:
Amniotic Fluid (Amniotic Fluid; Fetal Down Syndrome Testing)
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