Human Chemokine Array GS1
G-Series Human Chemokine Array 1 Kit. Semi-quantitative measurement of 40 human cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 16 Sample Kit, 32 Sample Kit, 64 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
6Ckine (CCL21)
| Axl
| Betacellulin (BTC)
| CCL28 (MEC)
| CTACK (CCL27)
|
CXCL16
| ENA-78 (CXCL5)
| Eotaxin-3 (CCL26)
| GCP-2 (CXCL6)
| GRO
|
HCC-1 (CCL14)
| HCC-4 (CCL16)
| IL-9
| IL-17F
| IL-18 BP alpha
|
IL-28A
| IL-29
| IL-31
| IP-10 (CXCL10)
| I-TAC (CXCL11)
|
LIF
| Light (TNFSF14)
| Lymphotactin (XCL1)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| MDC (CCL22)
| MIF
| MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
|
MPIF-1 (CCL23)
| MSP alpha/beta
| NAP-2 (PPBP/CXCL7)
| Osteopontin (SPP1)
| PARC (CCL18)
|
Platelet Factor 4 (CXCL4)
| SDF-1 alpha (CXCL12 alpha)
| TARC (CCL17)
| TECK (CCL25)
| TSLP
|
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Detection of Relative Protein Expression
- Detecting Patterns of Cytokine Expression
- Biomarker Screening
- Identifying Key Factors
- Confirming a Biological Process
Kit Components
- Antibody Array Slide/s
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
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Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with samples
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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