RayBio® G-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 43 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Apoptosis |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
bad
| bax
| bcl-2
| bcl-w
| BID
|
BIM
| Caspase-3
| Caspase-8
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
|
cIAP-2
| Cytochrome C | DR6 (TNFRSF21)
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
HSP27
| HSP60
| HSP70
| HTRA2
| IGF-1
|
IGF-2
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
IGFBP-5
| IGFBP-6
| IGF-1 R
| livin
| p21
|
p27
| p53
| SMAC
| Survivin (BIRC5)
| TNF RI (TNFRSF1A)
|
TNF RII (TNFRSF1B)
| TNF alpha
| TNF beta (TNFSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
|
TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| XIAP
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Apoptosis Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Protease Inhibitor Cocktail
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
The Presence of HIV-1 Tat Second Exon Delays Fas-Mediated Apoptosis in CD4+ T lymphocytes: a Potential Mechanism for Persistent Viral Production
Species:
Human
Sample Type:
Cell Lysate (Jurkat cell variants)
López-Huertas, MarÃÂa Rosa, et al. "The Presence of HIV-1 Tat Protein Second Exon Delays Fas Protein-mediated Apoptosis in CD4+ T Lymphocytes A POTENTIAL MECHANISM FOR PERSISTENT VIRAL PRODUCTION." Journal of Biological Chemistry 288.11 (2013): 7626-7644.
Species:
Human
Sample Type:
Cell Lysate (Jurkat-Tat101 and Jurkat-Tat72 cells)
Qin M., Luo Y., Meng X., et al. Myricitrin attenuates endothelial cell apoptosis to prevent atherosclerosis: An insight into PI3K/Akt activation and STAT3 signaling pathways. Vascul Pharmacol. 2015 Jul;70:23-34. doi: 10.1016/j.vph.2015.03.002
Species:
Human
Sample Type:
Cell Lysate (HUVEC Cells)
Asif M., Shafaei A., Jafari S., et al. Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study. Toxicol Lett. 2016 Aug 22;257:84-96. doi: 10.1016/j.toxlet.2016.05.027.
Species:
Human
Sample Type:
Cell Lysate (HCT116 colorectal carcinoma cells)
Ibrahim M., et al. ?-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-?B and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther. 2014 Sep 27;8:1629-47. doi: 10.2147/DDDT.S66105
Species:
Human
Sample Type:
Cell Lysate (Breast cancer cells, MCF-10 and MCF-7)
Li X., Liu X., Xu Y., et al. Expression profile of apoptotic and proliferative proteins in hypoxic HUVEC treated with statins. Int J Oncol. 2015 Feb;46(2):677-84. doi: 10.3892/ijo.2014.2780.
Species:
Human
Sample Type:
Cell Lysate (HUVEC Cells)
Chen, Yao, et al. "WAP four-disulfide core domain protein 2 gene (WFDC2) is a target of estrogen in ovarian cancer cells." Journal of ovarian research 9.1 (2016): 10.
Species:
Human
Sample Type:
Cell Lysate (Â HO8910-NA and HO8910-209 cells)
Sidhu P., et al. Anticancer activity of VDR-coregulator inhibitor PS121912. Cancer Chemotherapy and Pharmacology Published Online August 2014. DOI 10.1007/s00280-014-2549-y
Species:
Human
Sample Type:
Cell Lysate (HL-60 cells)
Mohidin T., Ng C. BARF1 gene silencing triggers caspase-dependent mitochondrial apoptosis in Epstein-Barr virus-positive malignant cells. J Biosci. 2015 Mar;40(1):41-51.
Species:
Human
Sample Type:
Cell Lysate (AG876 and Hone-Akata cells transfected with BARF1 siRNA)
Qin Y., Zhang S., Den S., et al. Epigenetic silencing of miR-137 induces drug resistance and chromosomal instability by targeting AURKA in multiple myeloma. Leukemia. 2017 Jan 6. doi: 10.1038/leu.2016.325.
Species:
Human
Sample Type:
Cell Lysate (H929 and mir-137 overexpressing cells)
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Great productMonitored protein expression in HMCB cells. Company’s free scanning of the array allowed us to use this tool and provide quality research experiences for undergraduates.
from Hiram College,
on
Great productA great assay for a fairly wide array of apoptosis proteins and if you lack the proper scanners the company will quickly scan and analyze your samples at no cost.from Columbia University,
on
Great productA very high-throughput, sensitive product to detect the apoptosis.on
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