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RayBio® Human HSP70 ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species
Human
Accession Number
P0DMV8
Gene Id
3308
Protein Name / Synonyms
Heat shock 70 kDa protein 1A (Heat shock 70 kDa protein 1) (HSP70-1) (HSP70.1)
Quantitative/Semi-Quantitative
Quantitative
Specificity
This ELISA antibody pair also can recognize mouse and rat HSP70
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Sandwich-based
Sensitivity
2 ng/mlNeed more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target . Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.
Detection Range
2 ng/ml - 600 ng/ml
Recommended Dilution (Serum/Plasma)
2 fold
Estimated Lead Time
1-2 business days
Shipping Type
Blue ice
Storage
≤-20°C
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
Learn More
Amazon Gift Cards!
$5 Amazon gift card in every kit box purchased.
Standard Curve
Spiking and Recovery Data
Linearity
Product Features
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Application Notes
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Pei, Gingshan, Hesheng Luo, and Xiaoping Zou. "Gemcitabine-treated Pancreatic Cancer Cell Medium Induces the Specific CTL Antitumor Activity by Stimulating the Maturation of Dendritic Cells." International Immunopharmacology. ScienceDirect, Mar. 2014. Web.
Sample Type:
Conditioned Media (Hsp70 protein levels of GEM-treated medium)
Great product
This ELISA kit is easy to use and has terrific results. This is the fourth time I’ve used it and will continue to do so.
Review by
Dan Huber,
from
The University of Tampa,
on
Mar 28, 2019
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