Quantibody® Human Angiogenesis Array 1000 Kit. A combination of Human Angiogenesis Array Q2 & Q3. Detects 60 Human Angiogenic Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 60 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Angiogenesis |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Activin A
| AgRP
| Angiopoietin-1
| Angiopoietin-2
| Angiogenin
|
Angiostatin
| ANGPTL4
| bFGF
| CXCL16
| EGF
|
ENA-78 (CXCL5)
| FGF-4
| Follistatin
| GCSF
| GM-CSF
|
GRO alpha/beta/gamma
| HB-EGF
| HGF
| I-309 (TCA-3/CCL1)
| IFN-gamma
|
IGF-1
| IL-10
| IL-12 p40
| IL-12 p70
| IL-17A
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-4
| IL-6
|
IL-8 (CXCL8)
| IP-10 (CXCL10)
| I-TAC (CXCL11)
| Leptin
| LIF
|
MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| MMP-1
|
MMP-9
| PDGF-BB
| PECAM-1 (CD31)
| PLGF
| RANTES (CCL5)
|
TGF alpha
| TGF beta 1
| TGF beta 3
| Tie-1
| Tie-2
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| Thrombopoietin (TPO)
|
uPAR
| VEGF-A
| VEGFR2
| VEGFR3
| VEGF-D
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Angiogenesis Array Q1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Gujar, Shashi A., et al. "Oncolytic virus-initiated protective immunity against prostate cancer." Molecular Therapy 19.4 (2011): 797-804.
Species:
Human
Sample Type:
Conditioned Media
Chen J, Liu Z, Hong MM, Zhang H, Chen C, et al. (2014) Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells. PLoS ONE 9(12): e115316. doi:10.1371/journal.pone.0115316
Species:
Human
Sample Type:
Cell Lysate (Microvesicles and MSC parents)
Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
Species:
Human
Sample Type:
Conditioned Media (Human Microvascular Endothelial Cells Culture Sup)
Storti, P., et al. "HOXB7 expression by myeloma cells regulates their pro-angiogenic properties in multiple myeloma patients." Leukemia 25.3 (2011): 527-537.
Species:
Human
Sample Type:
Cell Lysate
Zbucka-Kretowska, Monika, et al. "Amniotic fluid angiogenic and inflammatory factor profiling in foetal down syndrome." Fetal diagnosis and therapy 44 (2018): 44-50.
Species:
Human
Sample Type:
Amniotic fluid (Amniotic fluid for Down Syndrome)
Schuster C., Akslen L., Stokowy T., Straume O. Predictive value of angiogenic proteins in patients with metastatic melanoma treated with bevacizumab monotherapy. J Pathol Clin Res. 2019 Jan;5(1):53-62. doi: 10.1002/cjp2.116.
Species:
Human
Sample Type:
Serum (Biomarker of angiogenic proteins with metastatic melanoma and bevacizumab)
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Great productIt was hard to use at first but once you get the gist of how to use it, it becomes easier, I've been using it since 2017 to detect proteases on our artificial environment and works great! Recommended.
from NASA - Biology,
on
Great productThis product is simple to use with step by step instructions. It also has multiple options for an overnight incubation, which can be beneficial on a busy day!from UC Davis,
on
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