RayPlex Mouse Cytotoxic T Cell Bead Array 1
This RayPlex® kit is a cytometric bead array to quantitatively measure, by flow cytometry, 12 mouse cytokines associated with Cytotoxic T Cells (CTC). The assay has been optimized for serum and cell culture supernatant.
Product Description
Specifications
| Size | 48 tests, 96 tests, 192 tests, 480 tests |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 12 |
| Specificity | No detectable or significant cross reactivity was detected between antibodies or targets. |
| Compatible Sample Types | Cell Culture Supernatants, Serum |
| Solid Support | Fluorescent Microbeads |
| Method Of Detection | Flow Cytometry |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | 4°C |
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Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- RayPlex™ Multiplex Bead Cocktail
- 1X Assay Diluent
- 20X Wash Buffer
- RayBio® Serum Diluent
- Lyophilized Protein Standard Mix
- 1X Biotinylated Detection Antibody Cocktail
- 10X Streptavidin-PE
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
Other Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- 1.5 ml polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!
Protocol Outline
- Prepare Reagents, Standards, and Samples
- Add RayPlex™ Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.
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