RayBio® Mouse TNF alpha ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Mouse |
| Accession Number | P06804 |
| Gene Id | 21926 |
| Gene Symbols | TNF|TNFSF2 |
| Protein Name / Synonyms | Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form] |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 60 pg/ml (sensitivity of lysate is 94 pg/ml) Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 60 pg/ml - 6000 pg/ml (detection range of lysate is 94 pg/ml - 6000 pg/ml) |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Great ProductVery easy kit to use. I used tissue lysate, great consistent results. Highly recommend. Costumer service easy to reach and able to help with any questions.
from Boston Children’s Hospital ,
on
TNF-alpha lysateThe TNF-alpha lysate kit worked well and was simple to use. A quick assay.from Harvard,
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Great productThe cell lysate kit works well with my mice cells, the results are consistent. Good product.from Houston Methodist Research Institute,
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Great productThe kit works well and the instructions were easy to understand.from Houston Methodist Research Institute,
on
Finally! A tissue homogenate-compatible TNF ELISA!We've been looking for an ELISA kit that detects TNFa in tissue lysates for ages. We used this kit with mouse brain tissue homogenized in a lysis buffer containing 0.25% SDS and diluted the samples 1:5 as recommended by the manufacturer protocol and got beautiful results.from University of Illinois at Chicago,
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Great productThis Elisa kit is the way to go! You can even use just half the amount of the antibodies described in the manufacturer's protocol.from NanoBST,
on
Great productThis kit gave me great results with LPS-inflammed RAW264.7 cells supernatants. The instructions were clear and detailed and I like that you can use just half a plate and keep the other strips for another time. I would strongly recommend.from University of Arkansas,
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Great productI used the kit for cell culture supernatant analysis. The dilution times need yourself to investigate ahead and then do lots of experiments. Good products.from ALDERSON BROADDUS UNIVERSITY,
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Great productMouse spleen lysates and with denervated muscle tissue was a sample for use of the kit. There's very little reaction with lysates but it exists. From my experience, this kit works well for cell culture supernatants.from NanoBST,
on
Great productThe kit was packaged in an organized and clear manner, and it was easy to walk through each step of the procedure. I used the mouse IL-6, VEGF, and TNF-alpha kits for plasma as well as homogenized muscle samples. Each worked well, and I would be happy to use them again.from Houston Methodist Research Institute,
on
Great productThis kit worked very well for the analysis of mouse serum samples. It was shipped quickly, easy to use, and did exactly what it was supposed to. I highly recommend this product!from BTS Research,
on
Great productI used this ELISA kit for Serum and cell culture supernatant analysis. Great product!on
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