RayPlex Human Cytotoxic T Cell Bead Array 1
This RayPlex® kit is a cytometric bead array to quantitatively measure, by flow cytometry, 12 human cytokines associated with Cytotoxic T Cells (CTC). The assay has been optimized for serum and cell culture supernatant.
Product Description
Specifications
| Size | 192 tests, 48 tests, 480 tests, 96 tests |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 12 |
| Compatible Sample Types | Cell Culture Supernatants, Serum |
| Solid Support | Fluorescent Microbeads |
| Method Of Detection | Flow Cytometry |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | 4°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
| Granzyme A
| IFN-gamma
| IL-10
|
IL-17A
| IL-2
| IL-4
| IL-6
| TNF alpha
|
TNF beta (TNFSF1B)
| TRAIL (TNFSF10)
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- RayPlex™ Multiplex Bead Cocktail
- 1X Assay Diluent
- 20X Wash Buffer
- RayBio® Serum Diluent
- Lyophilized Protein Standard Mix
- 1X Biotinylated Detection Antibody Cocktail
- 10X Streptavidin-PE
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
Other Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- 1.5 ml polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!
Protocol Outline
- Prepare Reagents, Standards, and Samples
- Add RayPlex™ Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.
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