Detects 10 Rat Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Rat |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
ICAM-1 (CD54)
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-10
| IL-6
|
Leptin
| L-Selectin (CD62L)
| MCP-1 (CCL2)
| TIMP-1
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Rat Cytokine Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Zhou C., Fang X., Xu J., et al. Bifidobacterium longum alleviates irritable bowel syndrome-related visceral hypersensitivity and microbiota dysbiosis via Paneth cell regulation. Gut Microbes . 2020 Nov 9;12(1):1782156. doi: 10.1080/19490976.2020.1782156. Epub 2020 Jun 25
Species:
Rat
Sample Type:
Serum (Probiotic rat IBS model)
Driesen R., et al. Reversible and irreversible differentiation of cardiac fibroblasts. Cardiovasc Res. 2014 Mar 1;101(3):411-22. doi: 10.1093/cvr/cvt338
Species:
Rat
Sample Type:
Conditioned Media
Reiling, Janske, et al. "The role of macrophages in the development of biliary injury in a lipopolysaccharide-aggravated hepatic ischaemia-reperfusion model." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1864.4 (2018): 1284-1292.
Species:
Rat
Sample Type:
Bile (cholangiocytes)
Deuse T., Hua X., Taylor V., et al. Significant reduction of acute cardiac allograft rejection by selective janus kinase-1/3 inhibition using R507 and R545. Transplantation. 2012 Oct 15;94(7):695-702. doi: 10.1097/TP.0b013e3182660496.
Species:
Rat
Sample Type:
Tissue Lysate (Graft tissue lysate from heart tissue)
Narayanan, Ramesh, et al. "Discovery and preclinical characterization of novel small molecule TRK and ROS1 tyrosine kinase inhibitors for the treatment of cancer and inflammation." PLoS One 8.12 (2013): e83380.
Species:
Rat
Sample Type:
Air Pouch Exudates
Mishra R., Kovalska J., Janda J., et al. Tumor progression is associated with increasing CD11b+ cells and CCL2 in Lewis rat sarcoma. Anticancer Res. 2015 Feb;35(2):703-11
Species:
Rat
Sample Type:
Serum (Lewis Rat sarcoma model)
McMahon D, Oakden W, Hynynen K. Investigating the effects of dexamethasone on blood-brain barrier permeability and inflammatory response following focused ultrasound and microbubble exposure. Theranostics. 2020 Jan 1;10(4):1604-1618. doi: 10.7150/thno.40908. PMID: 32042325; PMCID: PMC6993222.
Species:
Rat
Sample Type:
Tissue Lysate
McMahon D, Oakden W, Hynynen K. Investigating the effects of dexamethasone on blood-brain barrier permeability and inflammatory response following focused ultrasound and microbubble exposure. Theranostics. 2020 Jan 1;10(4):1604-1618. doi: 10.7150/thno.40908. PMID: 32042325; PMCID: PMC6993222.
Species:
Rat
Sample Type:
Tissue Lysate
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