Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Rat
Accession Number	P14844
Gene Id	24770
Gene Symbols	CCL2\|SCYA2\|MCP1\|JE
Protein Name / Synonyms	C-C motif chemokine 2 (Immediate-early serum-responsive protein JE) (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Small-inducible cytokine A2)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	15 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	15 pg/ml - 18000 pg/ml
Recommended Dilution (Serum/Plasma)	500 - 10,000 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELR-MCP1.pdf)

Datasheet (ELR-MCP1-CL.pdf)

SDS (ELR-MCP1.pdf)

SDS (ELR-MCP1-CL.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Saleh, M. A., et al. "Endothelin receptor A-specific stimulation of glomerular inflammation and injury in a streptozotocin-induced rat model of diabetes." Diabetologia 54.4 (2011): 979-988.

Species:

Rat

Sample Type:

Serum

View Publication

Saleh, M. A., et al. "Endothelin receptor A-specific stimulation of glomerular inflammation and injury in a streptozotocin-induced rat model of diabetes." Diabetologia 54.4 (2011): 979-988.

Species:

Rat

Sample Type:

Tissue lysate (kidney homogenate)

View Publication

Yang D., Sun YY., Bhaumik SK., et al. Blocking lymphocyte trafficking with FTY720 prevents inflammation-sensitized hypoxic-ischemic brain injury in newborns. J Neurosci. 2014 Dec 3;34(49):16467-81. doi: 10.1523/JNEUROSCI.2582-14.2014

Species:

Rat

Sample Type:

Tissue Lysate (Rat Brain Tissue)

View Publication

Yamaleyeva LM, Guimaraes-Souza NK, Krane LS, et al. Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Stem Cells Translational Medicine. 2012;1(5):373-383. doi:10.5966/sctm.2011-0048

Species:

Rat

Sample Type:

Tissue Lysate (renal cortex and medulla of ischemia-reperfusion-gentamicin rats.)

View Publication

Yamaleyeva LM, Guimaraes-Souza NK, Krane LS, et al. Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Stem Cells Translational Medicine. 2012;1(5):373-383. doi:10.5966/sctm.2011-0048

Species:

Rat

Sample Type:

Urine (ischemia-reperfusion-gentamicin rats.)

View Publication

Yamaleyeva LM, Guimaraes-Souza NK, Krane LS, et al. Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Stem Cells Translational Medicine. 2012;1(5):373-383. doi:10.5966/sctm.2011-0048

Species:

Rat

Sample Type:

Urine (renal cortex and medulla of ischemia-reperfusion-gentamicin rats.)

View Publication

Wirrig C, Hunter I, Mathieson FA, Nixon GF. Sphingosylphosphorylcholine is a proinflammatory mediator in cerebral arteries. Journal of Cerebral Blood Flow & Metabolism. 2011;31(1):212-221. doi:10.1038/jcbfm.2010.79.

Species:

Rat

Sample Type:

Conditioned Media (A7r5 cells )

View Publication

Ikutomi M., Sahara M., Nakajima T., et al. Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation. J Mol Cell Cardiol. 2015 Sep;86:121-35. doi: 10.1016/j.yjmcc.2015.07.019.

Species:

Rat

Sample Type:

Conditioned Media (Endothelial progenitor cells, Rat endothelial aoritc cells, AoECS, and BM-derived mononuclear cells)

View Publication

Shen YC., et al. Dimemorfan protects rats against ischemic stroke through activation of sigma-1 receptor-mediated mechanisms by decreasing glutamate accumulation. J Neurochem. 2008 Jan;104(2):558-72. doi: 10.1111/j.1471-4159.2007.05058.x.

Species:

Rat

Sample Type:

Tissue Lysate (Brain tissue)

View Publication

Smits A., et al. Shear flow affects selective monocyte recruitment into MCP-1-loaded scaffolds. J. Cell. Mol. Med, Early Online view, August 2014. doi: 10.1111/jcmm.12330

Species:

Rat

Sample Type:

Conditioned Media

View Publication

View All Publications For This Product

Reviews

Write a customer review

under detection

Rating
20%

The assay was easy to follow, but could not detect (<0.0000) the anything from our samples (Rat casting-induced muscle atrophy). Used for plasma samples.

Review by Eunhee,

from University of Texas at San Antonio,

on May 26, 2023
Great product

Rating
100%

The kit is wonderful, I used Kidney homogenates.

Review by Riyasat Ali,

from University of Houston,

on May 14, 2020
Great product

Rating
100%

Excellent results with rat tissue homogenates and serum; easy to use; reproducible

Review by Kay Beharry,

from SUNY Downstate Medical Center,

on Jul 30, 2019
Great product

Rating
100%

Worked well for both plasma and tissue homogenate.

Review by Naureen,

from University of Houston,

on Jul 3, 2019

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