Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Rat
Accession Number	P29456
Gene Id	25325
Gene Symbols	IL10\|IL-10
Protein Name / Synonyms	Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	10 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	10 pg/ml - 6000 pg/ml
Recommended Dilution (Serum/Plasma)	2 - 10 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELR-IL10.pdf)

Datasheet (ELR-IL10-CL.pdf)

SDS (ELR-IL10.pdf)

SDS (ELR-IL10-CL.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Abdelkader NF., Safar MM., Salem H. Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson's Disease: Modulation of Mitochondrial Perturbations. Mol Neurobiol. 2014 Dec 15. [Epub ahead of print]. DOI 10.1007/s12035-014-9043-8

Species:

Rat

Sample Type:

Tissue Lysate (Striatal brain tissue samples)

View Publication

Mukherjee, Sanjib, et al. "Early TBI-induced cytokine alterations are similarly detected by two distinct methods of multiplex assay." Frontiers in molecular neuroscience 4 (2011): 21.

Species:

Rat

Sample Type:

Tissue Lysate (Brain homogenates)

View Publication

Chen H., Min XH., Wang QY., et al. Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury. Scientific Reports 5, Article number: 8718 (2015). doi:10.1038/srep08718

Species:

Rat

Sample Type:

Serum

View Publication

Chen H., Min XH., Wang QY., et al. Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury. Scientific Reports 5, Article number: 8718 (2015). doi:10.1038/srep08718

Species:

Rat

Sample Type:

Tissue Lysate (Intestinal Tissue lysates)

View Publication

Wazea, S.A., Wadie, W., Bahgat, A.K. et al. Galantamine anti-colitic effect: Role of alpha-7 nicotinic acetylcholine receptor in modulating Jak/STAT3, NF-?B/HMGB1/RAGE and p-AKT/Bcl-2 pathways. Sci Rep 8, 5110 (2018). https://doi.org/10.1038/s41598-018-23359-7

Species:

Rat

Sample Type:

Tissue Lysate (colon homogenate)

View Publication

Alhusban A., et al. Compound 21is pro-angiogenic in the brain and results in sustained recovery after ischemic stroke. Journal of Hypertension, October 2014. doi: 10.1097/HJH.0000000000000364

Species:

Rat

Sample Type:

Tissue Lysate (Brain tissue Lysate)

View Publication

Gu Y., Zhang Y., Bi Y., et al. Mesenchymal stem cells suppress neuronal apoptosis and decrease IL-10 release via the TLR2/NF?B pathway in rats with hypoxic-ischemic brain damage. Mol Brain. 2015 Oct 17;8(1):65. doi: 10.1186/s13041-015-0157-3.

Species:

Rat

Sample Type:

Tissue Lysate (Brain Tissue homogenate)

View Publication

Gu Y., Zhang Y., Bi Y., et al. Mesenchymal stem cells suppress neuronal apoptosis and decrease IL-10 release via the TLR2/NF?B pathway in rats with hypoxic-ischemic brain damage. Mol Brain. 2015 Oct 17;8(1):65. doi: 10.1186/s13041-015-0157-3.

Species:

Rat

Sample Type:

Conditioned Media (PC12 and other cell lines)

View Publication

Salga M., Ali H., Abdulla M., Abdelwahab S. Gastroprotective activity and mechanism of novel dichlorido-zinc(II)-4-(2-(5-methoxybenzylideneamino)ethyl)piperazin-1-iumphenolate complex on ethanol-induced gastric ulceration. Chem Biol Interact. 2012 Jan 25;195(2):144-53. doi: 10.1016/j.cbi.2011.11.008.

Species:

Rat

Sample Type:

Serum (Gastric ulceration model)

View Publication

Taha M., Salga M., Ali H., et al. Gastroprotective activities of Turnera diffusa Willd. ex Schult. revisited: Role of arbutin. J Ethnopharmacol. 2012 May 7;141(1):273-81. doi: 10.1016/j.jep.2012.02.030.

Species:

Rat

Sample Type:

Serum (Turnera diffusa treated animal in gastroprotection model)

View Publication

View All Publications For This Product

Reviews

Write a customer review

Great product

Rating
100%

The kit is wonderful, I used Kidney homogenates.

Review by Riyasat Ali,

from University of Houston,

on May 14, 2020
Great product

Rating
100%

The kit works so well as advertised, the protocol was clear to follow, would recommend the kit.

Review by Karthikeyan Thirugnanam,

from Medical College of Wisconsin,

on Aug 20, 2019
Great product

Rating
100%

Product is reliable and easy to use. Standard was very consistent and test samples were uniform and replicable. I like this product and have suggested my colleague in other lab. We are planning to purchase other cytokines kit as well.

Review by Sudhir Kumar,

on Mar 28, 2018
Great product

Rating
100%

I used this kit in a study with liver samples and it worked very well.

Review by Richa Rani,

on Mar 28, 2018

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