Mouse Neuro Discovery Array C1
C-Series Mouse Neuro Discovery Array C1 Kit. Detects 23 mouse neurologically relevant proteins on a membrane microarray. Suitable for serum, plasma, cell culture media and other liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 23 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | Neuroscience |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Fas Ligand
(TNFSF6)
|
Fractalkine
(CX3CL1)
|
GCSF
|
IFN-gamma
|
IGF-1
|
|
IL-10
|
IL-1 alpha
(IL-1 F1)
|
IL-1 beta
(IL-1 F2)
|
IL-4
|
IL-6
|
|
KC
(CXCL1)
|
LIX
|
MCP-1
(CCL2)
|
M-CSF
|
MIP-1 alpha
(CCL3)
|
|
MMP-2
|
MMP-3
|
RAGE
|
TARC
(CCL17)
|
SDF-1 alpha
(CXCL12 alpha)
|
|
TGF beta 1
|
TNF alpha
|
VEGF-A
|
||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Neuro Discovery C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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