Mouse Inflammation Array Q1
Quantibody® Mouse Inflammation Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 40 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Inflammation |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Fas Ligand (TNFSF6)
|
GCSF
| GM-CSF
| I-309 (TCA-3/CCL1)
| ICAM-1 (CD54)
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
| IL-12 p70
| IL-13
|
IL-15
| IL-17A
| IL-2
| IL-21
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-7
| KC (CXCL1)
|
Leptin
| LIX
| MCP-1 (CCL2)
| MCP-5
| M-CSF
|
MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 gamma
| Platelet Factor 4 (CXCL4)
| RANTES (CCL5)
|
TARC (CCL17)
| TIMP-1
| TNF alpha
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Sheedy, Frederick J., et al. "CD36 coordinates NLRP3 inflammasome activation by facilitating intracellular nucleation of soluble ligands into particulate ligands in sterile inflammation." Nature immunology 14.8 (2013): 812.
Species:
Mouse
Sample Type:
Serum (serile inflammation model)
Dou Y., Zhang X., Xu X., et al. Multiple noncovalent interactions mediated one-pot therapeutic assemblies for the effective treatment of atherosclerosis. J. Mater. Chem. B, 2015,3, 7355-7365, DOI: 10.1039/C5TB01474C
Species:
Mouse
Sample Type:
Serum (Artheroscelrosis mouse model)
Morin, C., Roumegous, A., Carpentier, G., Barbier-Chassefiere, V., Garrigue-Antar, L., Caredda, S., & Courty, J. (2012). Modulation of inflammation by Cicaderma ointment accelerates skin wound healing. Journal of Pharmacology and Experimental Therapeutics, 343(1), 115-124.
Species:
Mouse
Sample Type:
Tissue Lysate (Skin lysates, Mice injected with a doxorubicin solution)
Shi Y., Zhang L., Pu H., et al. Rapid endothelial cytoskeletal reorganization enables early blood-brain barrier disruption and long-term ischaemic reperfusion brain injury. Nat Commun. 2016 Jan 27;7:10523. doi: 10.1038/ncomms10523.
Species:
Mouse
Sample Type:
Tissue Lysate (Brain microvessels from the ipsilateral hemisphere)
Zeng Q., Chen W. The functional behavior of a macrophage/fibroblast co-culture model derived from normal and diabetic mice with a marine gelatineoxidized alginate hydrogel. Biomaterials 31 (2010) 5772-5781
Species:
Mouse
Sample Type:
Conditioned Media (Macrophages and Fibroblasts in Matrigel)
Zeng Q., et al. The functional behavior of a macrophage/fibroblast co-culture model derived from normal and diabetic mice with a marine gelatin-oxidized alginate hydrogel. Biomaterials. 2010 Aug;31(22):5772-81. doi: 10.1016/j.biomaterials.2010.04.022.
Species:
Mouse
Brod S. Ingested (oral) anti-IL-12/23 inhibits EAE. J Neurol Sci. 2016 Feb 15;361:19-25. doi: 10.1016/j.jns.2015.12.011.
Species:
Mouse
Sample Type:
Conditioned Media (MOG induced splenocyte repsonses)
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Love the cytokine arraysI used QAM-INF-1 for my mice plasma cytokine measurement. It worked really well and was cost-effective. I highly recommend it!
from Johns Hopkins University,
on
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