RayBio® G-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Inflammation |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
BLC
(CXCL13)
|
CD30 Ligand
(TNFSF8)
|
Eotaxin-1
(CCL11)
|
Eotaxin-2
(MPIF-2/CCL24)
|
Fas Ligand
(TNFSF6)
|
|
Fractalkine
(CX3CL1)
|
GCSF
|
GM-CSF
|
IFN-gamma
|
IL-1 alpha
(IL-1 F1)
|
|
IL-1 beta
(IL-1 F2)
|
IL-2
|
IL-3
|
IL-4
|
IL-6
|
|
IL-9
|
IL-10
|
IL-12 p40/p70
|
IL-12 p70
|
IL-13
|
|
IL-17A
|
I-TAC
(CXCL11)
|
KC
(CXCL1)
|
Leptin
|
LIX
|
|
Lymphotactin
(XCL1)
|
MCP-1
(CCL2)
|
M-CSF
|
MIG
(CXCL9)
|
MIP-1 alpha
(CCL3)
|
|
MIP-1 gamma
|
RANTES
(CCL5)
|
SDF-1 alpha
(CXCL12 alpha)
|
I-309
(TCA-3/CCL1)
|
TECK
(CCL25)
|
|
TIMP-1
|
TIMP-2
|
TNF alpha
|
TNF RI
(TNFRSF1A)
|
TNF RII
(TNFRSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Inflammation Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Zhou Y., Yu X., Chen H., et al. Leptin Deficiency Shifts Mast Cells toward Anti-Inflammatory Actions and Protects Mice from Obesity and Diabetes by Polarizing M2 Macrophages. Cell Metab. 2015 Dec 1;22(6):1045-58. doi: 10.1016/j.cmet.2015.09.013.
Species:
Mouse
Sample Type:
Conditioned Media (Leptin treated BMMC from wt and ob/ob mice)
Feng, Z., Qi, S., Zhang, Y. et al. Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury. Cell Death Dis 8, e3170 (2017). https://doi.org/10.1038/cddis.2017.549
Species:
Mouse
Sample Type:
BAL Fluid
Raghunatha, P., Vosoughi, A., Kauppinen, T. M., & Jackson, M. F. (2020). Microglial NMDA receptors drive pro?inflammatory responses via PARP?1/TRMP2 signaling. Glia. doi:10.1002/glia.23790Â
Species:
Mouse
Sample Type:
Conditioned media (cell culture media)
Chopy, Damien, et al. "Ambivalent role of the innate immune response in rabies virus pathogenesis." Journal of virology 85.13 (2011): 6657-6668.
Species:
Mouse
Sample Type:
Tissue Lysate (brain)
Coulombe G, Leblanc C, Cagnol S, et al. Epithelial Tyrosine Phosphatase SHP-2 Protects against Intestinal Inflammation in Mice. Mol Cell Biol. 2013;33(11):2275-2284.
Species:
Mouse
Sample Type:
Tissue Lysate (Colon protein extracts)
Huang Y., et al. Met-CCL5 represents an immunotherapy strategy to ameliorate rabies virus infection. Huang et al. Journal of Neuroinflammation 2014, 11:146. doi:10.1186/s12974-014-0146-y
Species:
Mouse
Sample Type:
Tissue Lysate (Brain tissue Lysate)
Keewan E, Narasimhulu CA, Rohr M, Hamid S, Parthasarathy S. Are Fried Foods Unhealthy? The Dietary Peroxidized Fatty Acid, 13-HPODE, Induces Intestinal Inflammation In Vitro and In Vivo. Antioxidants (Basel). 2020 Sep 27;9(10):926. doi: 10.3390/antiox9100926. PMID: 32992618; PMCID: PMC7601460.
Species:
Mouse
Sample Type:
Plasma
Liu X., Wu J., Wang M., et al. Identification of Novel Inflammatory Cytokines and Contribution of Keratinocyte-Derived Chemokine to Inflammation in Response to Vibrio vulnificus Infection in Mice. Inflammation. 2015 Oct;38(5):1864-73. doi: 10.1007/s10753-015-0166-5.
Species:
Mouse
Sample Type:
Serum (Serum from vibrio infected mice)
Liu X., Wu J., Wang M., et al. Identification of Novel Inflammatory Cytokines and Contribution of Keratinocyte-Derived Chemokine to Inflammation in Response to Vibrio vulnificus Infection in Mice. Inflammation. 2015 Oct;38(5):1864-73. doi: 10.1007/s10753-015-0166-5.
Species:
Mouse
Sample Type:
Tissue Lysate (Liver homogenate from vibrio infected mice)
Li SP, Wang FF, Zhang WK, Bian MZ, Zhang SY, Yan H, Fang Y, Zhang HM. Characteristics of Changes in Inflammatory Cytokines as a Function of Hepatic Ischemia-Reperfusion Injury Stage in Mice. Inflammation. 2019 Dec;42(6):2139-2147. doi: 10.1007/s10753-019-01078-y. PMID: 31494794.
Species:
Mouse
Sample Type:
Tissue Lysate (Liver)
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