Mouse Inflammation Array C1

Name: Mouse Inflammation Array C1
Brand: AAM-INF-1
SKU: AAM-INF-1
Price: 359 USD
Availability: InStock
Rating: 96 (9 reviews)

Rating:

96 % of 100

9 Reviews

57 Citations

RayBio^® C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.

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As low as $359.00

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Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Mouse
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	40
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	Membrane
Method Of Detection	Chemiluminescence
Design Principle	Sandwich-based
Research Area	Inflammation
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	-20°C

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Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA
Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA	IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA
IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-12 p40/p70 Gene Symbol: IL12A; IL12B Accession #: P43432 ELISA Kit: RayBio Mouse IL-12 p40/70 ELISA	IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA
IL-17A Gene Symbol: IL17A Accession #: Q60971	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA	Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA	LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA
Lymphotactin (XCL1) Gene Symbol: XCL1 Accession #: P47993 ELISA Kit: RayBio Mouse Lymphotactin ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA
MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Accession #: P40224 ELISA Kit: RayBio Mouse SDF-1 alpha ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA	TECK (CCL25) Gene Symbol: CCL25 Accession #: O35903 ELISA Kit: RayBio Mouse TECK ELISA
TIMP-1 Gene Symbol: TIMP1 Accession #: P12032 ELISA Kit: RayBio Mouse TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Accession #: Q6GXA8 ELISA Kit: RayBio Mouse TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Inflammation Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Documents

Datasheet (AAM-INF-1.pdf)

SDS (AAM-INF-1.pdf)

Excel (AAM-INF-1.xls)

Citations

Chien MH., Bien MY., Ku CC., et al. Systemic human orbital fat-derived stem/stromal cell transplantation ameliorates acute inflammation in lipopolysaccharide-induced acute lung injury. Crit Care Med. 2012 Apr;40(4):1245-53. doi: 10.1097/CCM.0b013e31823bc89a.

Species:

Mouse

Sample Type:

Serum (LPS treated)

View Publication

Patel P., Julien JP., Kriz J. Early-Stage Treatment with Withaferin A Reduces Levels of Misfolded Superoxide Dismutase 1 and Extends Lifespan in a Mouse Model of Amyotrophic Lateral Sclerosis. Neurotherapeutics. 2014 Nov 18. [Epub ahead of print]

Species:

Mouse

Sample Type:

Tissue Lysate (Spinal Cord lysates, withaferin A treatment)

View Publication

Rojewska E., Zychowska M., Piotrowska A., et al. Involvement of Macrophage Inflammatory Protein-1 Family Members in the Development of Diabetic Neuropathy and Their Contribution to Effectiveness of Morphine. Front Immunol. 2018 Mar 12;9:494. doi: 10.3389/fimmu.2018.00494.

Species:

Mouse

Sample Type:

Tissue Lysate (Spinal cord lysate from neuropathic diabetic mouse model)

View Publication

Lalancette-Hébert M., Swarup V., Beaulieu JM., et al. Galectin-3 is required for resident microglia activation and proliferation in response to ischemic injury. J Neurosci. 2012 Jul 25;32(30):10383-95. doi: 10.1523/JNEUROSCI.1498-12.2012

Species:

Mouse

Sample Type:

Tissue Lysate (Brain Lysate)

View Publication

Lalancette-Hébert, Mélanie, et al. "Accumulation of dietary docosahexaenoic acid in the brain attenuates acute immune response and development of postischemic neuronal damage." Stroke 42.10 (2011): 2903-2909.

Species:

Mouse

Sample Type:

Tissue Lysate (brain)

View Publication

Ke, F., Zhang, L. et al. Autocrine IL-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca2+ channels. Stem Cells 2014. Epub Ahead of Print. DOI: 10.1002/stem.1763

Species:

Mouse

Sample Type:

Conditioned Media (Skin-dervied Mesenchymal Stem Cells)

View Publication

Slesiona S., Ibrahim-Granet O., Olias P., et al. Murine infection models for Aspergillus terreus pulmonary aspergillosis reveal long-term persistence of conidia and liver degeneration. J Infect Dis. 2012 Apr 15;205(8):1268-77. doi: 10.1093/infdis/jis193.

Species:

Mouse

Sample Type:

Tissue Lysate (Organs, Liver, lung, others)

View Publication

Yamauchi, Keita, et al. "Attenuation of lung inflammation and fibrosis in CD69-deficient mice after intratracheal bleomycin." Respiratory research 12.1 (2011): 131.

Species:

Mouse

Sample Type:

BAL Fluid (bleomycin treated)

View Publication

Tang A., Li N., Li X., et al. Dynamic activation of the key pathways: linking colitis to colorectal cancer in a mouse model. Carcinogenesis. 2012 Jul;33(7):1375-83. doi: 10.1093/carcin/bgs183

Species:

Mouse

Sample Type:

Serum (Azoxymethane and dextran sulfate sodium treatment)

View Publication

Lou, You-Rong, et al. "Effects of high-fat diets rich in either omega-3 or omega-6 fatty acids on UVB-induced skin carcinogenesis in SKH-1 mice." Carcinogenesis 32.7 (2011): 1078-1084.

Species:

Mouse

Sample Type:

Tissue Lysate (epidermal tissue)

View Publication

View All Publications For This Product

Reviews

Write a customer review

Great product!!! Great results!!! Works well!!!

Rating
80%

Kit is cost effective and fulfill its porpose

Review by Inflammation Guy,

from University of Alabama at Birmingham,

on Sep 30, 2023
Great Product!

Rating
100%

Easy to use and a good range of cytokines! Would be helpful to add IP-10

Review by Anonymous,

from UConn,

on Aug 13, 2023
Great product

Rating
100%

Best way to evaluate quickly imflammatory cytokines in different tissues. Very easy to use. I advise different washes and revelation of all the membranes at the same time for 2 min between the 2 given plastic sheets. Would be even better to includes other cytokines like TGFb in the panel

Review by Matthias Dedobbeleer,

from Sickkids Hospital,

on Feb 26, 2023
Great product

Rating
80%

It is a quick way to observe the inflammatory cytokine and chemokine profile of mouse tumors. Multiple washes help reduce background noise.

Review by Anonymous,

from Ohio State University,

on Jul 20, 2022
Great product

Rating
100%

It's a quick inflammation array and really helpful. Hope to have more further discount in the future order.

Review by Robert Chiang,

from SUNY Downstate Health Sciences University,

on Jan 26, 2021
Great product

Rating
100%

The product is amazing. Following the manual, and make sure you wash enough (more buffer or more times) to make your array looking better.

Review by Nico,

from UPMC,

on May 11, 2020
Great product

Rating
100%

My first time using the protein array kit and I am very satisfied with the ease of the whole procedure as well as the results. I ordered 4 more membranes that I am satisfied with.

Review by Aicha Saadane,

from UCI,

on Dec 15, 2019
Great product

Rating
100%

Various fluids can be measured by this kit. It is important to select the protein concentration as a positive sample before the experiment. I recommend using a high sensitivity ECL solution for a clear image under the low background.

Review by Hyuk-Kwon Kwon,

from Yale University,

on Jun 27, 2019
Great product

Rating
100%

This kit costs less than others and covers most of inflammatory cytokines we need in the mouse model.

Review by Christina,

from Purdue University,

on May 22, 2018

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Featured products

Mouse Inflammation Array C1

Product Description

Specifications

Risk-Free Guarantee

Amazon Gift Cards!

Product Features

Target Names

Application Notes

Storage/Stability

Documents

Citations

Chien MH., Bien MY., Ku CC., et al. Systemic human orbital fat-derived stem/stromal cell transplantation ameliorates acute inflammation in lipopolysaccharide-induced acute lung injury. Crit Care Med. 2012 Apr;40(4):1245-53. doi: 10.1097/CCM.0b013e31823bc89a.

Patel P., Julien JP., Kriz J. Early-Stage Treatment with Withaferin A Reduces Levels of Misfolded Superoxide Dismutase 1 and Extends Lifespan in a Mouse Model of Amyotrophic Lateral Sclerosis. Neurotherapeutics. 2014 Nov 18. [Epub ahead of print]

Rojewska E., Zychowska M., Piotrowska A., et al. Involvement of Macrophage Inflammatory Protein-1 Family Members in the Development of Diabetic Neuropathy and Their Contribution to Effectiveness of Morphine. Front Immunol. 2018 Mar 12;9:494. doi: 10.3389/fimmu.2018.00494.

Lalancette-Hébert M., Swarup V., Beaulieu JM., et al. Galectin-3 is required for resident microglia activation and proliferation in response to ischemic injury. J Neurosci. 2012 Jul 25;32(30):10383-95. doi: 10.1523/JNEUROSCI.1498-12.2012

Lalancette-Hébert, Mélanie, et al. "Accumulation of dietary docosahexaenoic acid in the brain attenuates acute immune response and development of postischemic neuronal damage." Stroke 42.10 (2011): 2903-2909.

Ke, F., Zhang, L. et al. Autocrine IL-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca2+ channels. Stem Cells 2014. Epub Ahead of Print. DOI: 10.1002/stem.1763

Slesiona S., Ibrahim-Granet O., Olias P., et al. Murine infection models for Aspergillus terreus pulmonary aspergillosis reveal long-term persistence of conidia and liver degeneration. J Infect Dis. 2012 Apr 15;205(8):1268-77. doi: 10.1093/infdis/jis193.

Yamauchi, Keita, et al. "Attenuation of lung inflammation and fibrosis in CD69-deficient mice after intratracheal bleomycin." Respiratory research 12.1 (2011): 131.

Tang A., Li N., Li X., et al. Dynamic activation of the key pathways: linking colitis to colorectal cancer in a mouse model. Carcinogenesis. 2012 Jul;33(7):1375-83. doi: 10.1093/carcin/bgs183

Lou, You-Rong, et al. "Effects of high-fat diets rich in either omega-3 or omega-6 fatty acids on UVB-induced skin carcinogenesis in SKH-1 mice." Carcinogenesis 32.7 (2011): 1078-1084.

Reviews

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