RayBio® C-Series Human Th1/2/17 Antibody Array 1 Kit. Detects 34 Human Th1, Th2, and Th17 Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 34 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
CD30 (TNFRSF8)
| CD40 Ligand (TNFSF5)
| CD40 (TNFRSF5)
| GCSF
| GITR (TNFRSF18)
|
GM-CSF
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-1 R1
| IL-1 R2
|
IL-2
| IL-4
| IL-5
| IL-6
| IL-6 R
|
IL-10
| IL-12 p40
| IL-12 p70
| IL-13
| IL-17A
|
IL-17F
| IL-17 RA
| IL-21
| IL-21 R
| IL-22
|
IL-23
| IL-28A (IFN-lambda 2)
| MIP-3 alpha (CCL20)
| gp130
| TGF beta 1
|
TGF beta 3
| TNF alpha
| TNF beta (TNFSF1B)
| TRANCE (TNFSF11)
| |
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Th1/Th2/Th17 Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Chang M., Shen Y. Linalool Exhibits Cytotoxic Effects by Activating Antitumor Immunity. Molecules, 2014, 19, 6694-6706; doi:10.3390/molecules19056694
Species:
Human
Sample Type:
Conditioned Media (Human isolated lymphocytes cell)
Chen P, Chen Y, Jiang M, Mo Y, Ying H, Tang X, Zhang J. Usefulness of the cytokines expression of Th1/Th2/Th17 and urinary CD80 excretion in adult-onset minimal change disease. PeerJ. 2020 Sep 8;8:e9854. doi: 10.7717/peerj.9854. PMID: 33194357; PMCID: PMC7485503.
Species:
Human
Sample Type:
Serum
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