Quantitative, sandwich-based, glass slide antibody array to detect 30 human angiogenesis protein biomarkers.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 30 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Angiogenesis |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Activin A
| AgRP
| Angiogenin
| Angiopoietin-2
| ANGPTL4
|
bFGF
| ENA-78 (CXCL5)
| GRO alpha/beta/gamma
| HB-EGF
| HGF
|
IFN-gamma
| IGF-1
| IL-1 alpha (IL-1 F1)
| IL-2
| IL-6
|
IL-8 (CXCL8)
| IL-17A
| IP-10 (CXCL10)
| Leptin
| LIF
|
MCP-1 (CCL2)
| PDGF-BB
| PLGF
| RANTES (CCL5)
| TGF beta 1
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| Thrombopoietin (TPO)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Angiogenesis Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Das K., et al. Differential expression of steroid 5?-reductase isozymes and association with disease severity and angiogenic genes predict their biological role in prostate cancer. Endocr Relat Cancer. 2010 Aug 16;17(3):757-70. doi: 10.1677/ERC-10-0022.
Species:
Human
Sample Type:
Cell Lysate (cell lysate)
Das K., et al. Differential expression of steroid 5?-reductase isozymes and association with disease severity and angiogenic genes predict their biological role in prostate cancer. Endocr Relat Cancer. 2010 Aug 16;17(3):757-70. doi: 10.1677/ERC-10-0022.
Species:
Human
Xiu, G., Sun, J., Li, X., Jin, H., Zhu, Y., Zhou, X., Ling, B. (2018). The role of HMGB1 in BMSC transplantation for treating MODS in rats. Cell and Tissue Research, 373(2), 395-406. doi:10.1007/s00441-018-2823-0Â
Species:
Human
Sample Type:
Conditioned Media (cu;ltured BMSC cells)
Chen J, Liu Z, Hong MM, Zhang H, Chen C, et al. (2014) Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells. PLoS ONE 9(12): e115316. doi:10.1371/journal.pone.0115316
Species:
Human
Sample Type:
Cell Lysate (Microvesicles and MSC parents)
Schuster C., Akslen L., Stokowy T., Straume O. Predictive value of angiogenic proteins in patients with metastatic melanoma treated with bevacizumab monotherapy. J Pathol Clin Res. 2019 Jan;5(1):53-62. doi: 10.1002/cjp2.116.
Species:
Human
Sample Type:
Serum (Biomarker of angiogenic proteins with metastatic melanoma and bevacizumab)
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