Human Angiogenesis Array Q1
Quantitative, sandwich-based, glass slide antibody array to detect 10 human angiogenic factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Angiogenesis, HER/ErbB Signaling |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| Angiopoietin-2
| EGF
| bFGF
| HB-EGF
|
HGF
| Leptin
| PDGF-BB
| PLGF
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Angiogenesis Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
RodrÃÂguez?Muñoz, Y., et al. "Peripheral blood monocyte subsets predict antiviral response in chronic hepatitis C." Alimentary pharmacology & therapeutics 34.8 (2011): 960-971.
Species:
Human
Sample Type:
Conditioned Media (peripheral monocytes )
Numasawa Y., et al. Treatment of Human Mesenchymal Stem Cells with Angiotensin Receptor Blocker Improved Efficiency of Cardiomyogenic Transdifferentiation and Improved Cardiac Function via Angiogenesis. Stem Cells. 2011;29:1405-1414. DOI:10.1002/stem.691
Species:
Human
Sample Type:
Conditioned Media (BM dervied MSCs)
Shinmura D., et al. Pretreatment of Human Mesenchymal Stem Cells with Pioglitazone Improved Efficiency of Cardiomyogenic Transdifferentiation and Cardiac Function. STEM CELLS 2011;29:357-366. DOI:10.1002/stem.574
Species:
Human
Sample Type:
Conditioned Media (BM dervied MSCs)
Zheng, Yi, et al. "Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis." Advanced healthcare materials (2016).
Species:
Human
Sample Type:
Conditioned Media
Dao, Mo, et al. "Comparing the angiogenic potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells." Journal of translational medicine 11.1 (2013): 81.
Species:
Human
Sample Type:
Conditioned Media (MSCs and SB623 cells)
Pinto DS, Ahsan T, Serra J, Fernandes?Platzgummer A, Cabral JMS, da Silva CL. Modulation of the in vitro angiogenic potential of human mesenchymal stromal cells from different tissue sources. J Cell Physiol. 2020;1-15. https://doi.org/10.1002/jcp.29622
Species:
Human
Sample Type:
Conditioned media (UCM MSC culture media)
El Karim IA., et al. Neuropeptides Regulate Expression of Angiogenic Growth Factors in Human Dental Pulp Fibroblasts. J Endod. 2009 Jun;35(6):829-33. doi: 10.1016/j.joen.2009.03.005.
Species:
Human
Sample Type:
Conditioned Media (Dental pulp fibroblast-Conditioned Media)
El Karim IA., et al. Neuropeptides Regulate Expression of Angiogenic Growth Factors in Human Dental Pulp Fibroblasts. J Endod. 2009 Jun;35(6):829-33. doi: 10.1016/j.joen.2009.03.005.
Species:
Human
Landazuri N., Levit R., Joseph G., et al. Alginate microencapsulation of human mesenchymal stem cells as a strategy to enhance paracrine-mediated vascular recovery after hindlimb ischaemia. J Tissue Eng Regen Med. 2016 Mar;10(3):222-32. doi: 10.1002/term.1680.
Species:
Human
Sample Type:
Extracellular Vesicles (Capsules from MSCs contatining cytokines)
Maloney, Shawn C., et al. "Expression of SIRT1 in choroidal neovascular membranes." Retina 33.4 (2013): 862-866.
Species:
Human
Sample Type:
Conditioned Media (RPE-19 cells)
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Great productUsed this cytokine array for culture media from endothelial and cardiac cultures (roughly 100,000 cells in 300ul media). Diluted media 2x. The kit was very intuitive and easy to prepare (just time consuming with all the washes). I just wish this product came in a larger size on a single slide (such as a 96 well version) for higher throughout and efficiency, especially since the standards take up half the array wells, but it’s obviously working within the confines of the slide reader/format. It was difficult to prevent ‘comet’ shaped smears even while following the detailed wash protocol, but overall, the results came out quite nicely and this was a simple and useful assay.
from University of Toronto,
on
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