G-Series Human Angiogenesis Array 1 Kit. Semi-quantitative measurement of 10 human cytokines. Suitable for all liquid sample types.
Product Description
Specifications
Size | 16 Sample Kit, 32 Sample Kit, 64 Sample Kit |
---|---|
Species | Human |
Quantitative/Semi-Quantitative | Semi-Quantitative |
Number of Targets Detected | 10 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Glass Slide |
Method Of Detection | Fluorescence Laser Scanner |
Design Principle | Sandwich-based |
Research Area | Angiogenesis, HER/ErbB Signaling |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | -20°C |
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$5 Amazon gift card in every kit box purchased.
Scroll over each target protein for more information | ||||
---|---|---|---|---|
Angiogenin
| Angiopoietin-2
| EGF
| bFGF
| HB-EGF
|
HGF
| Leptin
| PDGF-BB
| PLGF
| VEGF-A
|
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Detection of Relative Protein Expression
- Detecting Patterns of Cytokine Expression
- Biomarker Screening
- Identifying Key Factors
- Confirming a Biological Process
Kit Components
- Antibody Array Slide/s
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
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Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with samples
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Cao B, Zhang N, Zhang Y, Fu Y, Zhao D. Plasma cytokines for predicting diabetic retinopathy among type 2 diabetic patients via machine learning algorithms. Aging (Albany NY). 2020 Dec 11;12. doi: 10.18632/aging.202168. Epub ahead of print. PMID: 33323553.
Species:
Human
Sample Type:
Plasma
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