Recombinant SARS-CoV-2 Nucleocapsid Protein, Biotinylated
Biotin-conjugated recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 100 ug |
|---|---|
| Species | SARS-CoV-2 |
| Accession Number | QHD43423 |
| Protein Name / Synonyms | Nucleocapsid Protein, N Protein |
| Expressed Region | Met1-Ala419 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The minor small protein bands (25-30 kDa) likely the cleaved products. See deglycosylation analysis image below. |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Estimated Lead Time | 2-3 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
