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Anti-Mouse MHC class I Antibody, RayBright® Blue 488

RayBright® Blue 488 Mouse MHC class I Antibody [clone: M1/42], monoclonal Rat IgG2a kappa. Validated for flow cytometry.
As low as $86.00
In stock
Only %1 left
Catalog #:
135-14034
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Product Description

Specifications

Size 25 µg, 100 µg
Species Mouse
Host Species Rat
Protein Name / Synonyms MHC class I
Clonality Monoclonal
Clone M1/42
Conjugation RayBright® Blue 488
Preparation The antibody was purified by affinity chromatography, and conjugated with a high quality fluorochrome, RayBright® Blue 488
Isotype IgG2a Kappa
Format Liquid
Concentration (lot specific) 0.5 mg/ml
Buffers PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide
Excitation Laser Blue Laser, 488 nm
Maximum Emission Wavelength 518 nm, replace FITC, Alexa Fluorâ„¢ 488, and BD BB515 (Alexa Fluorâ„¢ is the trademark of Invitrogen)
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage 4°C

Typical Data

Description

MHC class I molecules are expressed on the cell surface of all nucleated cells and platelets in mice. This antibody recognizes mouse H-2 of all haplotypes. MHC class I consists of three α units (α1, α2, and α3) and one β unit (β2 microglobulin). This complex interacts with CD8 T cell receptor complex and presents a non-self antigenic peptide which triggers an immediate response from the immune system against another non-self antigen.

Product Details

Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per 106 cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage/Stability
Stored at 2°C ~ 8°C, protected from prolonged light exposure. DO NOT FREEZE.
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