This antibody was produced from antiserum of rabbits immunized with a recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction was purified by Protein A affinity chromatography.
Recommended Applications
ELISA (recommended work dilution = 1:20,000)
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in 1X PBS, aliquot using ddH2O. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch.Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways.
Specificity
This antibody was selected for its ability to bind to the virus SARS-CoV-2 Nucleocapsid Protein from the disease COVID-19.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Expiration:
12 months from the date of shipment when stored properly.
