The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.
Recommended Applications
ELISA (recommended work dilution= 1: 8,000) , Western Blotting (recommended work dilution= 1: 1,000-5,000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant proteinIL-10. The membrane blot was probed with antiALPP primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein IL-10 from E.coli cell (19kDa).
Antigen Catalog Number
230-00242
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile 1x PBS to a final concentration of 1 mg/ml.
Interleukin 10(IL-10),which encoded by the IL10 gene is a cytokine with pleiotropic effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat IL-10 has not been tested yet.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -90°C. After reconstitution, it can be aliquoted and stored
References
Taras T Antoniv, et al. Interleukin-10-induced gene expression and suppressive function are selectively modulated by the PI3K-Akt-GSK3 pathway. Immunology. 2011 April; 132(4): 567–577.
Daniel E. Kaufmann et al. CD4+ CD25+ Regulatory T Cells Impair HIV-1-Specific CD4 T Cell Responses by Upregulating Interleukin-10 Production in Monocytes. J Virol. 2012 June; 86(12): 6586–6594.
Expiration:
12 months from the date of shipment when stored properly.
