Label-based antibody array for the relative detection of 2,808 mouse proteins on nitrocellulose membranes. Suitable for serum, plasma, lysates, and cell culture supernatants.
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
RayBio® L-Series Antibody Array Membranes
Labeling Reagent
Stop Solution
Blocking Buffer
Detection Buffer C
Detection Buffer D
Wash Buffer 1
Wash Buffer 2
HRP-Conjugated Streptavidin Concentrate
Plastic Sheets
Spin Columns
Labeling Buffer
Lysis Buffer
Plastic Incubation Tray
Other Materials Required
Distilled or de-ionized water
Small plastic or glass containers
Orbital shaker or oscillating rocker
Pipettors, pipette tips and other common lab consumables
2~5 ml tube
15 ml conical collection tube
Tissue Paper, blotting paper or chromatography paper
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
Purify Samples with Spin Column
Biotinylate Samples
Purify biotinylated samples using spin column
Block array membranes
Incubate with Samples
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Storage/Stability
For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
