Label-based antibody array for the relative detection of 2,808 mouse proteins on standard histology glass slides. Suitable for serum, plasma and cell culture supernatants.
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
RayBio® L-Series Antibody Array Glass Slide(s)
Spin Columns
Labeling Reagent
Stop Solution
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Streptavidin-Conjugated Fluor
Adhesive Plastic Strips
Labeling Buffer
Lysis Buffer
30 ml Centrifuge Tube
Other Materials Required
Distilled or de-ionized water
Small plastic or glass containers
Orbital shaker or oscillating rocker
1 ml tube
Pipettors, pipette tips and other common lab consumables
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
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Protocol Outline
Purify Samples with Spin Column
Biotinylate Samples
Purify biotinylated samples using spin column
Dry the glass slide
Block array surface
Incubate with Samples
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
